MT dynamic instability parameters in PtK1 cells
. | . | Control“Central” MTs . | Control“Pioneer” MTs . | PBD/ID(H83L) . | Rac1(Q61L) . | Rac1(Q61L) and PBD/ID(H83L) . | Rac1(T17N) . |
|---|---|---|---|---|---|---|---|
| Growth rate | Mean (μm/min) | 5.8 (± 2.7, n = 393) | 7.0 (± 3.6, n = 815) | 6.3 (± 3.2, n = 590) | 6.2 (± 2.7, n = 1043) | 5.2 (± 1.9, n = 457) | 6.3 (± 3.0, n = 396) |
| Median (μm/min) | 5.1 | 6.0 | 5.2 | 5.5 | 4.6 | 5.5 | |
| Shortening rate | Mean (μm/min) | −12.1 (± 8.4, n = 179) | −8.8 (± 7.5, n = 83) | −10.2 (± 5.7, n = 284) | −15.8 (± 12.7, n = 109) | −7.6 (± 5.2, n = 145) | −8.1 (± 3.8, n = 204) |
| Median (μm/min) | −10.1 | −6.0 | −9.3 | −11.5 | −6.0 | −7.4 | |
| Catastrophe frequency (s−1) | 0.052 (n = 103) | 0.014 (n = 59) | 0.047 (n = 139) | 0.014 (n = 73) | 0.044 (n = 101) | 0.058 (n = 115) | |
| Rescue frequency (s−1) | 0.109 (n = 98) | 0.145 (n = 60) | 0.096 (n = 136) | 0.129 (n = 70) | 0.149 (n = 108) | 0.110 (n = 112) | |
| Time spent | Growing (%) | 20.2 | 38.8 | 26.9 | 43.5 | 19.1 | 19.2 |
| Pausing (%) | 70.6 | 57.2 | 60.1 | 52.0 | 74.9 | 71.0 | |
| Shortening (%) | 9.2 | 3.9 | 12.9 | 4.5 | 6.1 | 9.9 | |
| Net growth | Mean (μm/min) | 0.0 (± 0.8) | 2.4 (± 1.2) | 0.2 (± 1.2) | 2.0 (± 1.2) | 0.5 (± 0.6) | 0.4 (± 0.7) |
| Total time observed (min) | 163 | 175 | 183 | 200 | 200 | 172 | |
| Number of cells, microtubules | 4, 35 | 4, 35 | 4, 36 | 4, 40 | 4, 40 | 5, 35 |
. | . | Control“Central” MTs . | Control“Pioneer” MTs . | PBD/ID(H83L) . | Rac1(Q61L) . | Rac1(Q61L) and PBD/ID(H83L) . | Rac1(T17N) . |
|---|---|---|---|---|---|---|---|
| Growth rate | Mean (μm/min) | 5.8 (± 2.7, n = 393) | 7.0 (± 3.6, n = 815) | 6.3 (± 3.2, n = 590) | 6.2 (± 2.7, n = 1043) | 5.2 (± 1.9, n = 457) | 6.3 (± 3.0, n = 396) |
| Median (μm/min) | 5.1 | 6.0 | 5.2 | 5.5 | 4.6 | 5.5 | |
| Shortening rate | Mean (μm/min) | −12.1 (± 8.4, n = 179) | −8.8 (± 7.5, n = 83) | −10.2 (± 5.7, n = 284) | −15.8 (± 12.7, n = 109) | −7.6 (± 5.2, n = 145) | −8.1 (± 3.8, n = 204) |
| Median (μm/min) | −10.1 | −6.0 | −9.3 | −11.5 | −6.0 | −7.4 | |
| Catastrophe frequency (s−1) | 0.052 (n = 103) | 0.014 (n = 59) | 0.047 (n = 139) | 0.014 (n = 73) | 0.044 (n = 101) | 0.058 (n = 115) | |
| Rescue frequency (s−1) | 0.109 (n = 98) | 0.145 (n = 60) | 0.096 (n = 136) | 0.129 (n = 70) | 0.149 (n = 108) | 0.110 (n = 112) | |
| Time spent | Growing (%) | 20.2 | 38.8 | 26.9 | 43.5 | 19.1 | 19.2 |
| Pausing (%) | 70.6 | 57.2 | 60.1 | 52.0 | 74.9 | 71.0 | |
| Shortening (%) | 9.2 | 3.9 | 12.9 | 4.5 | 6.1 | 9.9 | |
| Net growth | Mean (μm/min) | 0.0 (± 0.8) | 2.4 (± 1.2) | 0.2 (± 1.2) | 2.0 (± 1.2) | 0.5 (± 0.6) | 0.4 (± 0.7) |
| Total time observed (min) | 163 | 175 | 183 | 200 | 200 | 172 | |
| Number of cells, microtubules | 4, 35 | 4, 35 | 4, 36 | 4, 40 | 4, 40 | 5, 35 |
n, total number of events observed. ± indicates SD. In cells injected only with PBD/ID(H83L), dynamics parameters were determined from MTs in flat areas underneath neighboring cells. In all other conditions, MTs were analyzed in the free cell edge.