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Table I.

Adipose tissue ofperilipin-null animal contains HSL of normal specific activity


Animal

Wild type

perilipin-null
Densitometry
 (arbitrary units) 16,900 13,000 
Activity
 (nmol/h × mg protein−1686 518 
Specific activity
 
25%
 
25%
 

Animal

Wild type

perilipin-null
Densitometry
 (arbitrary units) 16,900 13,000 
Activity
 (nmol/h × mg protein−1686 518 
Specific activity
 
25%
 
25%
 

Aliquots of infranatants from adipose tissue of wt and perilipin-null mice were tested for lipolytic activity as described under Materials and methods. Equivalent cellular aliquots were electrophoresed under SDS-PAGE, im-munoblotted with affinity-purified anti-HSL, and scanned with a den-sitometer. The values for densitometric scans were divided by the activity values to give specific activities, revealing that the samples from both types of animals had identical specific activities.

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