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Table I.

Quantitation of cells containing activated MEK1 on Golgi membranes during mitosis


Stage

Fraction of cells containing
 phospho-MEK on Golgi membranes

Cells containing
 phospho-MEK on Golgi membranes


%
Interphase 0/50 
Early prophase 0/35 
Middle prophase 2/46 
Late prophase 24/29 83 
Prometaphase 4/50 
Metaphase to cytokinesis
 
0/50
 
0
 

Stage

Fraction of cells containing
 phospho-MEK on Golgi membranes

Cells containing
 phospho-MEK on Golgi membranes


%
Interphase 0/50 
Early prophase 0/35 
Middle prophase 2/46 
Late prophase 24/29 83 
Prometaphase 4/50 
Metaphase to cytokinesis
 
0/50
 
0
 

NRK cells treated as described in Fig. 4 were labeled for activated MEK1 and DNA and imaged by fluorescence microscopy. The mitotic stages were defined based on the level of chromosome condensation. The number of cells with activated MEK1 on the Golgi complex (numerator) and the number of cells counted (denominator) are shown. 83% of cells in late prophase contain activated MEK1 on Golgi membranes.

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