Fas ligation augments platelet production by MKs in a zVAD-fmk–inhibitable manner
Condition . | Proplatelet MKs per 2 × 105 MKs . | Functional platelet yield × 104 per 2 × 105 MKs . |
|---|---|---|
| Control | 1,193 ± 183 | 26 ± 3 |
| ZVAD-fmk (100 μM) | 5 ± 8a | 13 ± 1a |
| CH11 (50 ng/ml) | 8,024 ± 1,777a | 234 ± 38a |
| CH11;ZB4 (50 ng/ml; 1 μg/ml) | ND | 31 ± 2 |
| CH11;zVAD-fmk (50 ng/ml; 100 μM) | 784 ± 115a | 28 ± 4 |
| sFas-L (5 ng/ml) | ND | 134 ± 28a |
| sFas-L;zVAD-fmk (5 ng/ml; 100 μM) | ND | 29 ± 9 |
| TNFα (25 ng/ml) | ND | 30 ± 5 |
Condition . | Proplatelet MKs per 2 × 105 MKs . | Functional platelet yield × 104 per 2 × 105 MKs . |
|---|---|---|
| Control | 1,193 ± 183 | 26 ± 3 |
| ZVAD-fmk (100 μM) | 5 ± 8a | 13 ± 1a |
| CH11 (50 ng/ml) | 8,024 ± 1,777a | 234 ± 38a |
| CH11;ZB4 (50 ng/ml; 1 μg/ml) | ND | 31 ± 2 |
| CH11;zVAD-fmk (50 ng/ml; 100 μM) | 784 ± 115a | 28 ± 4 |
| sFas-L (5 ng/ml) | ND | 134 ± 28a |
| sFas-L;zVAD-fmk (5 ng/ml; 100 μM) | ND | 29 ± 9 |
| TNFα (25 ng/ml) | ND | 30 ± 5 |
P < 0.01 compared to control.
Proplatelet MK count represents total number of proplatelet-bearing MKs following 8 h of culture, scored by phase microscopy. Functional platelet yield represents total number of platelets produced following 18 h of culture, enumerated as detailed in Fig. 3. CH11 is an anti-Fas agonistic mAb, and ZB4 an antagonistic mAb. Data represent mean ± SD of a minimum of three determinations.