Altering the activity of myosin, RhoA and MLCK does not alter growth cone F-actin content
Treatment . | Mean growth cone f-actin content in arbitrary units + SEM (n) . |
|---|---|
| BSA (control) | 1263 ± 163 (30) |
| Skmyosin II | 1167 ± 142 (32) |
| MLCKp1 | 1192 ± 97 (43) |
| C3 | 1184 ± 171 (26) |
| CaRhoA | 1114 ± 158 (56) |
Treatment . | Mean growth cone f-actin content in arbitrary units + SEM (n) . |
|---|---|
| BSA (control) | 1263 ± 163 (30) |
| Skmyosin II | 1167 ± 142 (32) |
| MLCKp1 | 1192 ± 97 (43) |
| C3 | 1184 ± 171 (26) |
| CaRhoA | 1114 ± 158 (56) |
Dorsal root ganglion neurons were trituration loaded with protein or peptide, cultured for 3 h and then fixed and stained with phalloidin. No statistically significant differences from control trituration loaded growth cones were observed.