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Table I.

Recovery of EGFP–topoIIα after photobleaching at kinetochores and chromosome arms


Structure

t1/2 s (SD)

Range

% recovery (SD)

Range

n
Kinetochore 15.7 (4.7) 9.0–22.4 61.9 (14.7) 42.8–86.7 
Chromosome arm
 
15.5 (3.6)
 
9.9–22.5
 
75.9 (13.0)
 
52.4 – 90.0
 
10
 

Structure

t1/2 s (SD)

Range

% recovery (SD)

Range

n
Kinetochore 15.7 (4.7) 9.0–22.4 61.9 (14.7) 42.8–86.7 
Chromosome arm
 
15.5 (3.6)
 
9.9–22.5
 
75.9 (13.0)
 
52.4 – 90.0
 
10
 

Kinetochores and chromosome arms of GT2-LPk cells were photobleached with a nitrogen dye laser. Recovery of fluorescence was measured in the photobleached area from images taken at intervals after photobleaching. After correcting for background and loss of fluorescence due to image capture, measurements were used to calculate halftime recovery (t 1/2) and the percentage of recovery.

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