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Table I.

Progression of microinfected cells through mitosis


Injection

Cell phase
 at injection

Number of cells
 injected

Number of cells
 arrested at prometaphase

Number of cells
 arrested at metaphase

Number of cells
 that completed mitosis
Buffer Prophase   
 Prometaphase   
Cdc34CA (≥3 mg/mL) Prophase 11   11 
 Prometaphase   
Cdc34 (≥3 mg/mL) Prophase 104 89 10 
 Prometaphase 24 18 
 Late prometaphase 11  7* 
 Anaphase   
Cdc34 (3 mg/mL) denatured Prophase   
 Prometaphase   
Cdc34 (1.5 mg/mL) Prophase 11  
Cdc34 (3 mg/mL) with
 10 μm MG132 or β-lactone
 
Prophase
 
15
 
15
 

 

 

Injection

Cell phase
 at injection

Number of cells
 injected

Number of cells
 arrested at prometaphase

Number of cells
 arrested at metaphase

Number of cells
 that completed mitosis
Buffer Prophase   
 Prometaphase   
Cdc34CA (≥3 mg/mL) Prophase 11   11 
 Prometaphase   
Cdc34 (≥3 mg/mL) Prophase 104 89 10 
 Prometaphase 24 18 
 Late prometaphase 11  7* 
 Anaphase   
Cdc34 (3 mg/mL) denatured Prophase   
 Prometaphase   
Cdc34 (1.5 mg/mL) Prophase 11  
Cdc34 (3 mg/mL) with
 10 μm MG132 or β-lactone
 
Prophase
 
15
 
15
 

 

 
*

These cells spent on average 32.5 min at metaphase, which is significantly different from uninjected cells (P < 0.05).

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