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Table II.

Evidence for the role of NFAT in cellular adaptation


Cell type

NFAT isoform implicated

Methods used to determine role for NFAT

Target gene

Function

References
Pancreatic islet cells (cell line) NFATC2 EMSA and reporter assays1 Glucagon Expression of glucagon Furstenau et al., 1999  
Epidermal cell
 (cell line) Reporter assays2 in vitro  and in vivo Response to UV radiation Huang et al., 2000  
Cardiac muscle NFATC4 Overexpression in  transgenic mice Cardiac hypertrophy Molkentin et al., 1998  
Skeletal myofibers (in vitro) NFATC1 Nuclear localization Response to nerve stimuli Liu et al., 2001  
Skeletal muscle cells (cell line) Reporter assays3 MyHC II Expression of MyHC II isoforms Allen et al., 2001  
VSMCs
 (primary cells) NFATC1/C2 Reporter assays4 Agonist induced signaling Boss et al., 1998  
ISMCs
 (isolated tissue)
 
NFATC3
 
Nuclear localization;  reporter assays5
 
?
 
Response to PDGF
 
Stevenson et al., 2001 
 

Cell type

NFAT isoform implicated

Methods used to determine role for NFAT

Target gene

Function

References
Pancreatic islet cells (cell line) NFATC2 EMSA and reporter assays1 Glucagon Expression of glucagon Furstenau et al., 1999  
Epidermal cell
 (cell line) Reporter assays2 in vitro  and in vivo Response to UV radiation Huang et al., 2000  
Cardiac muscle NFATC4 Overexpression in  transgenic mice Cardiac hypertrophy Molkentin et al., 1998  
Skeletal myofibers (in vitro) NFATC1 Nuclear localization Response to nerve stimuli Liu et al., 2001  
Skeletal muscle cells (cell line) Reporter assays3 MyHC II Expression of MyHC II isoforms Allen et al., 2001  
VSMCs
 (primary cells) NFATC1/C2 Reporter assays4 Agonist induced signaling Boss et al., 1998  
ISMCs
 (isolated tissue)
 
NFATC3
 
Nuclear localization;  reporter assays5
 
?
 
Response to PDGF
 
Stevenson et al., 2001 
 
1

Glucagon promoter constructs.

2

NFAT reporter construct; NFAT-luciferase transgenic mice.

3

MyHC II promoter constructs.

4

NFAT reporter construct.

5

NFAT-luciferase transgenic mice.

EMSA, electrophoretic mobility shift assay.

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