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Table II.

Characterization of pauses in droplet motion




Pause duration

Time between pauses

Percentage reversals associated with a pause
ss
Pauses after minus-end travel
 

 

 

 
wild-type (*) 0.62 ± 0.03 5.03 ± 0.31 13.8 
Dhc64C6-10/Dhc64C8-1 (*)
 
0.76 ± 0.03
 
4.24 ± 0.31
 
17.4
 
Dhc64C6-10/+
 
0.62 ± 0.03
 
3.99 ± 0.29
 
14.2
 
Dhc64C8-1/+
 
0.70 ± 0.03
 
2.98 ± 0.18
 
17.5
 
Gl1/+
 
0.64 ± 0.04
 
4.24 ± 0.31
 
13.4
 
Pauses after plus-end travel
 

 

 

 
wild-type (*)
 
0.55 ± 0.20
 
7.63 ± 0.45
 
11.7
 
Dhc64C6-10/Dhc64C8-1
 
0.71 ± 0.03
 
3.98 ± 0.19
 
18.7
 
Dhc64C6-10/+
 
0.60 ± 0.03
 
5.90 ± 0.32
 
12.7
 
Dhc64C8-1/+
 
0.71 ± 0.04
 
3.94 ± 0.23
 
16.7
 
Gl1/+
 
0.65 ± 0.04
 
4.90 ± 0.30
 
13.7
 



Pause duration

Time between pauses

Percentage reversals associated with a pause
ss
Pauses after minus-end travel
 

 

 

 
wild-type (*) 0.62 ± 0.03 5.03 ± 0.31 13.8 
Dhc64C6-10/Dhc64C8-1 (*)
 
0.76 ± 0.03
 
4.24 ± 0.31
 
17.4
 
Dhc64C6-10/+
 
0.62 ± 0.03
 
3.99 ± 0.29
 
14.2
 
Dhc64C8-1/+
 
0.70 ± 0.03
 
2.98 ± 0.18
 
17.5
 
Gl1/+
 
0.64 ± 0.04
 
4.24 ± 0.31
 
13.4
 
Pauses after plus-end travel
 

 

 

 
wild-type (*)
 
0.55 ± 0.20
 
7.63 ± 0.45
 
11.7
 
Dhc64C6-10/Dhc64C8-1
 
0.71 ± 0.03
 
3.98 ± 0.19
 
18.7
 
Dhc64C6-10/+
 
0.60 ± 0.03
 
5.90 ± 0.32
 
12.7
 
Dhc64C8-1/+
 
0.71 ± 0.04
 
3.94 ± 0.23
 
16.7
 
Gl1/+
 
0.65 ± 0.04
 
4.90 ± 0.30
 
13.7
 

Droplet motion in phase II embryos was characterized by centroid tracking. Pauses after minus-end or plus-end travel were recognized automatically by custom software and quantitated as described previously (Gross et al., 2000; Materials and methods). (First column) Average pause duration. (Second column) Time between pauses, a measure for how frequently pauses occur. (Third column) Fraction of reversals associated with a pause. This parameter indicates whether droplets typically pause first before reversing travel direction. In all cases, a majority of reversals occurs without a detectable pause. The data in rows labeled (*) are from our previous analysis of droplet travel (Gross et al., 2000) and are included for comparison.

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