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Table I.

Physical parameters of droplet motion in various genetic backgrounds




Mean travel
 distance

Distance constant
 short–slow state DS

Distance constant
 long–fast state DL

χv2, P(χv2)

Number ratio RSL

nm
nm
nm


Minus end      
wild-type (*)  620 ± 31 98 ± 8 1068 ± 149 1.20, 0.19 2.15 ± 0.59 
Dhc64C6-10/Dhc64C8-1(*)  168 ± 8 44 ± 4 209 ± 15 0.41, 0.97 2.25 ± 0.58 
Dhc64C6-10/ 494 ± 25 100 ± 6 903 ± 133 1.09, 0.37 1.50 ± 0.50 
Dhc64C8-1/ 287 ± 19 91 ± 7 648 ± 167 0.86, 0.73 4.44 ± 2.09 
Gl1/+  370 ± 28 52 ± 7 379 ± 54 0.97, 0.59 2.42 ± 0.77 
Plus end      
wild-type (*)  842 ± 35 67 ± 6 1144 ± 140 0.98, 0.51 1.05 ± 0.22 
Dhc64C6-10/Dhc64C8-1  311 ± 18 52 ± 4 440 ± 41 0.92, 0.61 1.26 ± 0.41 
Dhc64C6-10/ 557 ± 25 88 ± 7 783 ± 64 0.69, 0.85 2.42 ± 0.69 
Dhc64C8-1/ 377 ± 21 74 ± 9 402 ± 40 0.72, 0.91 1.45 ± 0.41 
Gl1/+
 
 403 ± 26
 
75 ± 8
 
583 ± 91
 
0.89, 0.70
 
2.44 ± 0.82
 



Mean travel
 distance

Distance constant
 short–slow state DS

Distance constant
 long–fast state DL

χv2, P(χv2)

Number ratio RSL

nm
nm
nm


Minus end      
wild-type (*)  620 ± 31 98 ± 8 1068 ± 149 1.20, 0.19 2.15 ± 0.59 
Dhc64C6-10/Dhc64C8-1(*)  168 ± 8 44 ± 4 209 ± 15 0.41, 0.97 2.25 ± 0.58 
Dhc64C6-10/ 494 ± 25 100 ± 6 903 ± 133 1.09, 0.37 1.50 ± 0.50 
Dhc64C8-1/ 287 ± 19 91 ± 7 648 ± 167 0.86, 0.73 4.44 ± 2.09 
Gl1/+  370 ± 28 52 ± 7 379 ± 54 0.97, 0.59 2.42 ± 0.77 
Plus end      
wild-type (*)  842 ± 35 67 ± 6 1144 ± 140 0.98, 0.51 1.05 ± 0.22 
Dhc64C6-10/Dhc64C8-1  311 ± 18 52 ± 4 440 ± 41 0.92, 0.61 1.26 ± 0.41 
Dhc64C6-10/ 557 ± 25 88 ± 7 783 ± 64 0.69, 0.85 2.42 ± 0.69 
Dhc64C8-1/ 377 ± 21 74 ± 9 402 ± 40 0.72, 0.91 1.45 ± 0.41 
Gl1/+
 
 403 ± 26
 
75 ± 8
 
583 ± 91
 
0.89, 0.70
 
2.44 ± 0.82
 

Droplet motion in phase II embryos was characterized by centroid tracking and statistical analysis of travel distances as described (Gross et al., 2000). To characterize the two travel states, histograms of travel distance, D, like the ones in Figs. 5 and 6, were fitted to the sum of two exponential functions: y(D) = AS exp(−D/DS) + AL exp(−D/DL). χν2 values and corresponding probabilities indicate the goodness of this fit. DS and DL measure the average travel distance of runs in the short–slow and the long–fast travel state, respectively. The number ratio RSL is the number of short runs divided by the number of long runs, and thus measures the relative frequency of the two travel states (see Gross et al., 2000 and Materials and methods for details regarding how these parameters are derived). The data in rows labeled (*) are from our previous analysis of droplet travel (Gross et al., 2000) and are included for comparison.

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