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Table I.

The effect of 43-kD SPDO overexpression during myofibril assembly


Developmental stage when IFM myofibrils fixed

Heat shock

Sarcomere length

Thin filament length

Core thin filament length

Myofibril diameter (DZ)

Core myofibril diameter

Core volume/total

DZ/DH

 
time
 
μm
 
μm
 
μm
 
μm
 
μm
 
%
 
%
 
yw (controls)         
Adult − 3.53 ± .08 1.49 ± 0.08  1.12 ± 0.09   98 ± 7 
Adult D4 APF 3.44 ± 0.1 1.45 ± 0.07  1.14 ± 0.11   98 ± 8 
hs-43 kD spdo         
Pupal D4 APF − 3.01 ± 0.12 1.25 ± 0.07  0.63 ± 0.14    
Pupal D4 APF D4 APF 2.91 ± 0.1 1.21 ± 0.06  0.62 ± 0.03    
Adult − 3.55 ± 0.10 1.52 ± 0.04  1.19 ± 0.09   98 ± 3 
Adult D3 APF 3.42 ± 0.05 1.44 ± 0.04 1.06 ± 0.06 1.14 ± 0.06 0.32 ± 0.05 5.3 ± 2 98 ± 4 
Adult D4 APF 3.48 ± 0.12 1.52 ± 0.07 1.28 ± 0.03 1.11 ± 0.04 0.62 ± 0.08 24.0 ± 6 98 ± 5 
Adult D1 Adult 3.44 ± 0.12 1.42 ± 0.06  1.23 ± 0.08   98 ± 4 
Adult 1 D D4 APF 3.42 ± 0.22 1.42 ± 0.10 1.16 ± 0.05 0.99 ± 0.14 0.43 ± 0.15   
Adult 10 D
 
D4 APF
 
3.50 ± 0.16
 
1.50 ± 0.08
 
1.18 ± 0.04
 
1.06 ± 0.12
 
0.48 ± 0.12
 

 

 

Developmental stage when IFM myofibrils fixed

Heat shock

Sarcomere length

Thin filament length

Core thin filament length

Myofibril diameter (DZ)

Core myofibril diameter

Core volume/total

DZ/DH

 
time
 
μm
 
μm
 
μm
 
μm
 
μm
 
%
 
%
 
yw (controls)         
Adult − 3.53 ± .08 1.49 ± 0.08  1.12 ± 0.09   98 ± 7 
Adult D4 APF 3.44 ± 0.1 1.45 ± 0.07  1.14 ± 0.11   98 ± 8 
hs-43 kD spdo         
Pupal D4 APF − 3.01 ± 0.12 1.25 ± 0.07  0.63 ± 0.14    
Pupal D4 APF D4 APF 2.91 ± 0.1 1.21 ± 0.06  0.62 ± 0.03    
Adult − 3.55 ± 0.10 1.52 ± 0.04  1.19 ± 0.09   98 ± 3 
Adult D3 APF 3.42 ± 0.05 1.44 ± 0.04 1.06 ± 0.06 1.14 ± 0.06 0.32 ± 0.05 5.3 ± 2 98 ± 4 
Adult D4 APF 3.48 ± 0.12 1.52 ± 0.07 1.28 ± 0.03 1.11 ± 0.04 0.62 ± 0.08 24.0 ± 6 98 ± 5 
Adult D1 Adult 3.44 ± 0.12 1.42 ± 0.06  1.23 ± 0.08   98 ± 4 
Adult 1 D D4 APF 3.42 ± 0.22 1.42 ± 0.10 1.16 ± 0.05 0.99 ± 0.14 0.43 ± 0.15   
Adult 10 D
 
D4 APF
 
3.50 ± 0.16
 
1.50 ± 0.08
 
1.18 ± 0.04
 
1.06 ± 0.12
 
0.48 ± 0.12
 

 

 

Myofibril parameters were measured from deconvolved images. ±, SEM; Dz, diameter measured at the Z-discs; DH, diamerter measured at the H zone; n, 15 myofibrils measured from three separate experiments for 2–3 day adult flies unless otherwise indicated.

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