Skip to Main Content
Table I.

ILK-binding proteins


Binding protein

ILK-binding site

Target on ILK

Detectiona
Transmembrane receptors    
(1) β1 integrins β1 cytoplasmic domain Kinase domain 2-hybrid, co-IP (Hannigan et al., 1996) 
(2) β3 integrins Not determined Not determined Co-IP (Hannigan et al., 1996) 
Adaptor proteins    
(1) PINCH LIM1 ANK domain Co-IP (Hannigan et al., 1996), ELISA (Tu et al., 1999) 
(2) CH-ILKBP CH2 Kinase domain 2-hybrid, co-IP, pull-down (Tu et al., 2001) 
(3) Affixin CH2 Kinase domain 2-hybrid, co-IP (Yamaji et al., 2001) 
(4) Paxillin LD1 Kinase domain Co-IP, pull-down (Nikolopoulos and Turner, 2001) 
Catalytic proteins    
(1) ILKAP Not determined ANK-PH domains 2-hybrid, co-IP (Leung-Hagesteijn et al., 2001) 
(2) PKB/Akt Not determined Kinase domain Co-IP (Persad et al., 2001a) 
(3) PDK-1
 
Not determined
 
Not determined
 
Co-IP (Persad et al., 2001a)
 

Binding protein

ILK-binding site

Target on ILK

Detectiona
Transmembrane receptors    
(1) β1 integrins β1 cytoplasmic domain Kinase domain 2-hybrid, co-IP (Hannigan et al., 1996) 
(2) β3 integrins Not determined Not determined Co-IP (Hannigan et al., 1996) 
Adaptor proteins    
(1) PINCH LIM1 ANK domain Co-IP (Hannigan et al., 1996), ELISA (Tu et al., 1999) 
(2) CH-ILKBP CH2 Kinase domain 2-hybrid, co-IP, pull-down (Tu et al., 2001) 
(3) Affixin CH2 Kinase domain 2-hybrid, co-IP (Yamaji et al., 2001) 
(4) Paxillin LD1 Kinase domain Co-IP, pull-down (Nikolopoulos and Turner, 2001) 
Catalytic proteins    
(1) ILKAP Not determined ANK-PH domains 2-hybrid, co-IP (Leung-Hagesteijn et al., 2001) 
(2) PKB/Akt Not determined Kinase domain Co-IP (Persad et al., 2001a) 
(3) PDK-1
 
Not determined
 
Not determined
 
Co-IP (Persad et al., 2001a)
 
a

2-hybrid, yeast two-hybrid binding assays; co-IP, coimmunoprecipitation; pull-down, GST fusion protein pull-down assay.

Close Modal

or Create an Account

Close Modal
Close Modal