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Table I.

LDH activity in various polysomal fractions


Fraction

Control(n = 12)

COL(n = 4)

Cy-D(n = 8)

3′UTR(n = 12)

Myoblast(n = 7)
Free 95.0 ± 0.6 90.0 ± 3.7 95.1 ± 0.8 93.1 ± 1.0 93.2 ± 1.5 
Cytoskeletal 3.3 ± 0.6 9.0 ± 3.8 3.5 ± 0.5 4.1 ± 0.8 3.9 ± 1.1 
Membrane
 
0.7 ± 0.2
 
1.1 ± 0.6
 
1.4 ± 0.4
 
3.0 ± 0.5
 
3.0 ± 0.5
 

Fraction

Control(n = 12)

COL(n = 4)

Cy-D(n = 8)

3′UTR(n = 12)

Myoblast(n = 7)
Free 95.0 ± 0.6 90.0 ± 3.7 95.1 ± 0.8 93.1 ± 1.0 93.2 ± 1.5 
Cytoskeletal 3.3 ± 0.6 9.0 ± 3.8 3.5 ± 0.5 4.1 ± 0.8 3.9 ± 1.1 
Membrane
 
0.7 ± 0.2
 
1.1 ± 0.6
 
1.4 ± 0.4
 
3.0 ± 0.5
 
3.0 ± 0.5
 

Aliquots from each polysomal fraction were assayed for LDH. As expected, the preferential activity of LDH within the free (cytosolic) polysomal fraction. Data are expressed as a percent of the total activity. The heading for each column represents the different experiments performed in this study, i.e., control myotubes, myotubes treated with COL or Cy-D, myotubes transfected with reporter constructs containing the utrophin 3′UTR, and myoblasts used for examining the effects of differentiation.

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