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Table III.

Summary of double step eventsb


Myosin


Mean displacement

Displacement duration

Displacement variance

Normalized variance

 

 
nm
 
ms
 
nm2
 

 
M5HMM Step 1 19 ± 1 213 ± 38 55 ± 3 0.58 ± 0.02 
 Step 2 18 ± 1 687 ± 132a 64 ± 4a 0.68 ± 0.03a 
 Unitary steps 18 ± 1 192 ± 17 59 ± 1 0.61 ± 0.01 
 Step 1 12 ± 1 468 ± 134 26 ± 2 0.58 ± 0.04 

 
Step 2
 
9 ± 2
 
400 ± 95
 
17 ± 1a
 
0.38 ± 0.01a
 

Myosin


Mean displacement

Displacement duration

Displacement variance

Normalized variance

 

 
nm
 
ms
 
nm2
 

 
M5HMM Step 1 19 ± 1 213 ± 38 55 ± 3 0.58 ± 0.02 
 Step 2 18 ± 1 687 ± 132a 64 ± 4a 0.68 ± 0.03a 
 Unitary steps 18 ± 1 192 ± 17 59 ± 1 0.61 ± 0.01 
 Step 1 12 ± 1 468 ± 134 26 ± 2 0.58 ± 0.04 

 
Step 2
 
9 ± 2
 
400 ± 95
 
17 ± 1a
 
0.38 ± 0.01a
 
a

Significant differences between step 1 and 2 of each myosin type was determined by Student's t test (p < 0.01).

b

The myosin II used was a baculovirus-expressed (–)insert isoform of smooth muscle myosin HMM (Lauzon et al., 1998) with experiments done at an ATP concentration of 10 μM (n = 25 events). Displacement amplitude, duration, and variance were calculated from hand-tallied events.

Values are means ± SEM. M5HMM double steps, n = 59 events; unitary steps, n = 357 events. Normalized variance is the ratio of event variance to baseline variance for that experiment.

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