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Table I.

Summary of in vitro motility and laser trap data


Heavy chain construct

Light chain composition

Velocity

Unitary displacement

Displacement duration


μm s−1
nm
ms
M5HMM Calmodulin 0.41 ± 0.06a 21 ± 2 (6) 120 ± 12 (6) 
M5HMM Calmodulin/LC1sa 0.30 ± 0.07 18 ± 2 (5) 111 ± 31 (5) 
M5HMM(tag) Calmodulin 0.46 ± 0.04a 18 ± 2 (5) 100 ± 13 (14) 
M5HMM(tag)
 
Calmodulin/LC1sa
 
0.26 ± 0.06
 
24 ± 2 (6)
 
138 ± 21 (5)
 

Heavy chain construct

Light chain composition

Velocity

Unitary displacement

Displacement duration


μm s−1
nm
ms
M5HMM Calmodulin 0.41 ± 0.06a 21 ± 2 (6) 120 ± 12 (6) 
M5HMM Calmodulin/LC1sa 0.30 ± 0.07 18 ± 2 (5) 111 ± 31 (5) 
M5HMM(tag) Calmodulin 0.46 ± 0.04a 18 ± 2 (5) 100 ± 13 (14) 
M5HMM(tag)
 
Calmodulin/LC1sa
 
0.26 ± 0.06
 
24 ± 2 (6)
 
138 ± 21 (5)
 
a

p < 0.01 when the effects of light chain composition were compared for a given heavy chain construct.

The in vitro motility values are means ± SD at 25 mM KCl. The unitary displacement values are means ± SEM. The quantities in parentheses represent the number of MV histograms (i.e., data sets) that contribute to the mean. LC1sa refers to the nonmuscle essential light chain.

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