Measurement of GJC in cell clones containing wt or mutant cx43 genes with or without the v-src gene
Cell clone . | wt or mutant cx43 . | v-src . | GJC . | Incidence of coupling . |
|---|---|---|---|---|
| % | ||||
| wtC1 | wt | − | 13.1 ± 0.5 (24) | 100 |
| wtC2 | wt | − | 13.9 ± 0.9 (23) | 100 |
| wtS1 | wt | + | 1.3 ± 0.2 (53) | 19 |
| wtS2 | wt | + | 1.0 ± 0.2 (45) | 18 |
| 247FC1 | Y247F | − | 10.3 ± 0.5 (15) | 100 |
| 247FS1 | Y247F | + | 10.4 ± 0.8 (45) | 98 |
| 247FS2 | Y247F | + | 11.8 ± 0.4 (43) | 100 |
| 265FC1 | Y265F | − | 10.7 ± 0.8 (17) | 100 |
| 265FC2 | Y265F | − | 12.5 ± 0.5 (32) | 100 |
| 265FS1 | Y265F | + | 9.0 ± 0.6 (39) | 97 |
| 265FS2 | Y265F | + | 8.6 ± 0.6 (31) | 100 |
| dbC1 | Y247F/Y265F | − | 11.3 ± 0.6 (28) | 100 |
| dbC2 | Y247F/Y265F | − | 9.8 ± 0.6 (42) | 95 |
| dbS1 | Y247F/Y265F | + | 12.7 ± 0.7 (39) | 100 |
| dbS2 | Y247F/Y265F | + | 9.6 ± 0.6 (37) | 100 |
Cell clone . | wt or mutant cx43 . | v-src . | GJC . | Incidence of coupling . |
|---|---|---|---|---|
| % | ||||
| wtC1 | wt | − | 13.1 ± 0.5 (24) | 100 |
| wtC2 | wt | − | 13.9 ± 0.9 (23) | 100 |
| wtS1 | wt | + | 1.3 ± 0.2 (53) | 19 |
| wtS2 | wt | + | 1.0 ± 0.2 (45) | 18 |
| 247FC1 | Y247F | − | 10.3 ± 0.5 (15) | 100 |
| 247FS1 | Y247F | + | 10.4 ± 0.8 (45) | 98 |
| 247FS2 | Y247F | + | 11.8 ± 0.4 (43) | 100 |
| 265FC1 | Y265F | − | 10.7 ± 0.8 (17) | 100 |
| 265FC2 | Y265F | − | 12.5 ± 0.5 (32) | 100 |
| 265FS1 | Y265F | + | 9.0 ± 0.6 (39) | 97 |
| 265FS2 | Y265F | + | 8.6 ± 0.6 (31) | 100 |
| dbC1 | Y247F/Y265F | − | 11.3 ± 0.6 (28) | 100 |
| dbC2 | Y247F/Y265F | − | 9.8 ± 0.6 (42) | 95 |
| dbS1 | Y247F/Y265F | + | 12.7 ± 0.7 (39) | 100 |
| dbS2 | Y247F/Y265F | + | 9.6 ± 0.6 (37) | 100 |
The nomenclature of the cell clones, the different cx43 genes, and whether the v-src gene is expressed are indicated. GJC values were measured by transfer of Lucifer yellow dye following the microinjection of single cells. The number of adjacent cells that become fluorescent (± SEM) was counted 1 min after microinjection. The total numbers of cells injected for each clone are indicated in parentheses. The percentages of injected cells that transferred dye to more than one neighboring cell are recorded as the incidence of coupling. Except for the wt cells (P < 0.01), there was no significant difference in the values of GJC between the v-Src containing clones and the non–v-Src–containing clones (P > 0.05 in a two-tailed Student's t test).