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Table I.

Genotype analysis of Uba3+/intercross progeny


Genotype
DNA source
+/+
+/−
−/−
ND
Resorbed
Tails (4-wk old) 97 163   
Yolk sacs (E11.5–17.5)  
Embryos (E7.5–E8.5) 12  
Blastocysts (E3.5) 17 30  
Blastocysts (E3.5 cultured)      
Normal ICM  
Atrophied ICM
 
0
 
0
 
2
 

 

 

Genotype
DNA source
+/+
+/−
−/−
ND
Resorbed
Tails (4-wk old) 97 163   
Yolk sacs (E11.5–17.5)  
Embryos (E7.5–E8.5) 12  
Blastocysts (E3.5) 17 30  
Blastocysts (E3.5 cultured)      
Normal ICM  
Atrophied ICM
 
0
 
0
 
2
 

 

 

Genotype analysis of Uba3 heterozygous intercross progeny. Embryos were genotyped by Southern blotting or PCR analysis (Fig. 1, A–C). Empty decidua were scored as “Resorbed.” Some blastocysts harvested at E3.5 were grown in culture before recovering DNA for genotyping. Phenotype data of cultured blastocysts represents typical data from one set of experiments. ND, not determined.

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