Effect of Different B-type Cyclin–CDKs on Subcellular Architecture
| . | 3–4 h . | 6–6.5 h . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Injection | Minimum number of experiments | Disassembled Golgi | Aster-like microtubules | Solubilized lamina | Condensed chromosome | Minimum number of experiments | Disassembled Golgi | Aster-like microtubules | Solubilized lamina | Condensed chromosomes |
| % (n) | % (n) | |||||||||
| Cyclin B1 + CDK1AF | 4 | 92 (89) | 28 (95) | 81 (59) | 36 (59) | 3 | 95 (103) | 63 (70) | 89 (82) | 89 (82) |
| Cyclin B2 + CDK1AF | 4 | 88 (73) | 0 (26) | 0.6 (47) | 0 (27) | 3 | 90 (120) | 1 (53) | 1 (31) | 1 (31) |
| CDK1AF | 3 | 15 (79) | 3 (30) | 2 (49) | 2 (49) | 2 | 12 (74) | 6 (30) | 0 (44) | 0 (44) |
| B1–B2 chimera + CDK1AF | 3 | 90 (54) | 39 (51) | 2 (48) | 2 (48) | 2 | 93 (48) | 67 (44) | 0 (31) | 0 (31) |
| B2–B1 chimera + CDK1AF | 3 | 88 (94) | 8 (49) | 2 (45) | 2 (48) | 2 | 96 (49) | 0 (22) | 0 (28) | 0 (28) |
| . | 3–4 h . | 6–6.5 h . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Injection | Minimum number of experiments | Disassembled Golgi | Aster-like microtubules | Solubilized lamina | Condensed chromosome | Minimum number of experiments | Disassembled Golgi | Aster-like microtubules | Solubilized lamina | Condensed chromosomes |
| % (n) | % (n) | |||||||||
| Cyclin B1 + CDK1AF | 4 | 92 (89) | 28 (95) | 81 (59) | 36 (59) | 3 | 95 (103) | 63 (70) | 89 (82) | 89 (82) |
| Cyclin B2 + CDK1AF | 4 | 88 (73) | 0 (26) | 0.6 (47) | 0 (27) | 3 | 90 (120) | 1 (53) | 1 (31) | 1 (31) |
| CDK1AF | 3 | 15 (79) | 3 (30) | 2 (49) | 2 (49) | 2 | 12 (74) | 6 (30) | 0 (44) | 0 (44) |
| B1–B2 chimera + CDK1AF | 3 | 90 (54) | 39 (51) | 2 (48) | 2 (48) | 2 | 93 (48) | 67 (44) | 0 (31) | 0 (31) |
| B2–B1 chimera + CDK1AF | 3 | 88 (94) | 8 (49) | 2 (45) | 2 (48) | 2 | 96 (49) | 0 (22) | 0 (28) | 0 (28) |
The percentage of cells with disassembled Golgi apparatus, microtubules reorganized to form asters, solubilized nuclear lamins, and condensed chromosomes at 3–3.5 and 6–6.5 h after injection with specific cyclins and CDK constucts are given along with the number of cells scored as positive for the injection marker.