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Table 1

Measured Parameters of S. pombe Interphase MT Dynamics

MTs, cells
n
MT growth rate Vg   
High GFP-tubulin expression level 1.86 ± 0.54 μm min−1 67, 26 
Low GFP-tubulin expression level 1.90 ± 0.33 μm min−1 7, 4 
Before tip contact 2.08 ± 0.53 μm min−1 17, 10 
After tip contact 1.30 ± 0.44 μm min−1 17, 10 
Average maximum ratio 1.35 ± 0.33 31, 23 
MT shrinkage rate Vs   
High GFP-tubulin expression level 8.99 ± 2.82 μm min−1 64, 26 
Low GFP-tubulin expression level 9.50 ± 3.85 μm min−1 7, 4 
MT catastrophe frequency fcat 0.31 min−1 49, 19 
MT rescue frequency fres 0 min−1 49, 19 
Duration of MT cell tip contact before catastrophe 1.50 ± 0.59 min 179, 33 
MTs, cells
n
MT growth rate Vg   
High GFP-tubulin expression level 1.86 ± 0.54 μm min−1 67, 26 
Low GFP-tubulin expression level 1.90 ± 0.33 μm min−1 7, 4 
Before tip contact 2.08 ± 0.53 μm min−1 17, 10 
After tip contact 1.30 ± 0.44 μm min−1 17, 10 
Average maximum ratio 1.35 ± 0.33 31, 23 
MT shrinkage rate Vs   
High GFP-tubulin expression level 8.99 ± 2.82 μm min−1 64, 26 
Low GFP-tubulin expression level 9.50 ± 3.85 μm min−1 7, 4 
MT catastrophe frequency fcat 0.31 min−1 49, 19 
MT rescue frequency fres 0 min−1 49, 19 
Duration of MT cell tip contact before catastrophe 1.50 ± 0.59 min 179, 33 

Rates were determined by linear regression analysis of each MT analyzed over 5–20 displacements measured 2–5 s apart. Data are given as mean ± SD of the rates measured for each MT, with n = number of MTs and cells measured.

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