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Table 2

The Differentiation Signal Acts Across a Short Distance

Embryoid bodies containing mutant cellsState of mutant cells in the embryoid body
TotalDifferentiatedUndifferentiated
Nonfused 145 145 (100%) 
Fused 86 29 (33.7%) 57 (66.3%) 
Total 231   
Embryoid bodies containing mutant cellsState of mutant cells in the embryoid body
TotalDifferentiatedUndifferentiated
Nonfused 145 145 (100%) 
Fused 86 29 (33.7%) 57 (66.3%) 
Total 231   

Primary aggregates (5–20 cells) of mutant and wild-type ES cells were mixed and grown for 6 d and embryoid bodies containing LaZ-positive differentiated or undifferentiated cells were counted.

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