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Table 1

Antiserum Specificity for the Small Tim Proteins

Radiolabeled protein
Antisera35S-Tim8p35S-Tim9p35S-Tim10p35S-Tim13p
Preimmune 0.06 0.22 0.04 < 0.01 
α-Tim8p 29.4 0.16 < 0.01 0.01 
α-Tim9p 0.10 11.75 0.22 0.01 
α-Tim10p 0.03 0.21 38.6 0.01 
α-Tim12p 0.02 0.01 0.14 < 0.01 
α-Tim13p 0.01 0.01 0.02 17.4 
Radiolabeled protein
Antisera35S-Tim8p35S-Tim9p35S-Tim10p35S-Tim13p
Preimmune 0.06 0.22 0.04 < 0.01 
α-Tim8p 29.4 0.16 < 0.01 0.01 
α-Tim9p 0.10 11.75 0.22 0.01 
α-Tim10p 0.03 0.21 38.6 0.01 
α-Tim12p 0.02 0.01 0.14 < 0.01 
α-Tim13p 0.01 0.01 0.02 17.4 

5 μl of 35S-labeled Tim8p, Tim9p, Tim10p, or Tim13p was added to 100 μg of mitochondria and solubilized in 100 μl boiling buffer. Samples were incubated at 95°C for 5 min, diluted with 1 ml TNET, and immunoprecipitated as described in Materials and Methods with the indicated antiserum. Values shown are the percentage of total TIM protein added to each reaction that was precipitated with the corresponding antiserum.

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