Rockefeller University Press

Table 2

Comparison Lucifer Yellow Protocols to Study GJIC in Early Xenopus Embryos

Study	Measured Lucifer transfer at stages	How passage via cytoplasmic bridges was excluded	All data werecollected from	Control for reflectionand scattered light
	No. of cells
Warner et al. 1984	32	No fluorescent dextran injections	Whole-mounts
Guthrie 1984	32	Lucifer yellow was injected into one group of	Whole-mounts	Few sibling embryos were
Guthrie et al. 1988	16–128	embryos and fluorescent dextran was injected	Whole-mounts	analyzed on frozen or plastic sections
Olson et al. 1991	32	a separate group of embryos	Whole-mounts	No sections
Nagajski et al. 1989	32	No fluorescent dextran injections	Whole-mounts	No sections
Olson and Moon 1992	32	Lucifer yellow was injected into one group of	Whole-mounts	No sections
Guger and Gumbiner 1995	32	embryos and fluorescent dextran was injected into	Whole-mounts	No sections
Krufka et al. 1998	32	a separate group of embryos	Whole-mounts	No sections
Levin and Mercola 1998	8–16	Fluorescent dextran was injected in a mixture solution with Lucifer yellow	Whole-mounts	No sections
This study	16–128	Fluorescent dextran was injected in a mixture solution with Lucifer yellow	Paraffin or frozen sections	All embryos were analyzed on either 12- μm paraffin or 14-μm frozen sections.

Study	Measured Lucifer transfer at stages	How passage via cytoplasmic bridges was excluded	All data werecollected from	Control for reflectionand scattered light
	No. of cells
Warner et al. 1984	32	No fluorescent dextran injections	Whole-mounts
Guthrie 1984	32	Lucifer yellow was injected into one group of	Whole-mounts	Few sibling embryos were
Guthrie et al. 1988	16–128	embryos and fluorescent dextran was injected	Whole-mounts	analyzed on frozen or plastic sections
Olson et al. 1991	32	a separate group of embryos	Whole-mounts	No sections
Nagajski et al. 1989	32	No fluorescent dextran injections	Whole-mounts	No sections
Olson and Moon 1992	32	Lucifer yellow was injected into one group of	Whole-mounts	No sections
Guger and Gumbiner 1995	32	embryos and fluorescent dextran was injected into	Whole-mounts	No sections
Krufka et al. 1998	32	a separate group of embryos	Whole-mounts	No sections
Levin and Mercola 1998	8–16	Fluorescent dextran was injected in a mixture solution with Lucifer yellow	Whole-mounts	No sections
This study	16–128	Fluorescent dextran was injected in a mixture solution with Lucifer yellow	Paraffin or frozen sections	All embryos were analyzed on either 12- μm paraffin or 14-μm frozen sections.