Rockefeller University Press

Table 1

Equal Neurobiotin Transfer in Dorsally or Ventrally Injected 64-Cell Stage Embryos

Embryo	Dorsal	Ventral
1	87	66
2	98	123
3	59	48
4	143	100
5	131	85
6	72	129
7	112	107
8	92	116
9	103	71
10	127	52
11	61	111
12	120	88
Mean	100.4	91.3
SD	27.5	27.4

Embryo	Dorsal	Ventral
1	87	66
2	98	123
3	59	48
4	143	100
5	131	85
6	72	129
7	112	107
8	92	116
9	103	71
10	127	52
11	61	111
12	120	88
Mean	100.4	91.3
SD	27.5	27.4

12 dorsally injected and 12 ventrally injected embryos were each analyzed on 70 serial sections each 12 μm thick. Fluorescent cell profiles were counted on each section for both neurobiotin and dextran probes, and only the neurobiotin-positive and dextran-negative cells were scored positively. The results show no significant difference in neurobiotin transfer between dorsally or ventrally injected embryos.

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