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Table III

Mutants Defective in bad15 Display a Daughter-specific Defect in Bud-site Selection

% Class I % Class II % Class III
Relevant genotype
graphic
% Class IV Other n
1. α BAD15    98     1    <1    <1    200 
2. aBAD15/BAD15     7    33    53      600 
3. α bad15    21    71     5      300 
4. abad15/bad15     9    20    63      300 
5. α bad43    22    51    21      100 
6. α bud4    10    27    40    24    300 
                     
Relevant genotype    % Class I    % Classes II, III, IV     
7. a1bad15/+    93     7    100 
8. a1bad15/bad15    15    85    100 
9. a1bad43/bad15    12    88    100 
% Class I % Class II % Class III
Relevant genotype
graphic
% Class IV Other n
1. α BAD15    98     1    <1    <1    200 
2. aBAD15/BAD15     7    33    53      600 
3. α bad15    21    71     5      300 
4. abad15/bad15     9    20    63      300 
5. α bad43    22    51    21      100 
6. α bud4    10    27    40    24    300 
                     
Relevant genotype    % Class I    % Classes II, III, IV     
7. a1bad15/+    93     7    100 
8. a1bad15/bad15    15    85    100 
9. a1bad43/bad15    12    88    100 

Budding pattern was determined by analyzing the morphology of microcolonies (see Materials and Methods). Class I, axial pattern, mother and daughter cells budded adjacent to the previous division site; Class II, mother cells budded near the previous division site, daughter cells budded distal to the previous division site; Class III, both mother and daughter cells budded distal to the previous division site; Class IV, all other microcolony morphologies. Class II and III together represent the bipolar budding pattern. The mother cell is drawn on the left. Strains used were: SY77, line 1; SY204, line 2; SY167, line 3; SY202, line 4; SY172, line 5; SY171, line 6; SY221 × IH2531, line 7; SY221 × SY167, line 8; and SY221 × SY172, line 9.  

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