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Table 1

Distribution of Centrosomes in CACO-2 Cells after Treatments that Disrupt the Cytoskeleton

% Centrosomes per region
Condition % Total cells Apical Deep cytoplasmic Lateral 
Anticytoskeletal agents     
None (control) N/A 91 
Nocodazole N/A 98 
Cytochalasin D N/A 94 
Acrylamide N/A 67 33 
Acrylamide reversal (24 h) N/A 87 13 
Anti-CK19 antisense oligonucleotides    
A19     
Nontargeted cells 39 98 
Partially targeted cells 54 36 36 28 
Fully targeted cells 56 44 
A19/2     
Nontargeted cells 36 92 
Partially targeted cells 62 41 33 26 
Fully targeted cells 50 50 
% Centrosomes per region
Condition % Total cells Apical Deep cytoplasmic Lateral 
Anticytoskeletal agents     
None (control) N/A 91 
Nocodazole N/A 98 
Cytochalasin D N/A 94 
Acrylamide N/A 67 33 
Acrylamide reversal (24 h) N/A 87 13 
Anti-CK19 antisense oligonucleotides    
A19     
Nontargeted cells 39 98 
Partially targeted cells 54 36 36 28 
Fully targeted cells 56 44 
A19/2     
Nontargeted cells 36 92 
Partially targeted cells 62 41 33 26 
Fully targeted cells 50 50 

CACO-2 cells (9 d stage) were either treated with anticytoskeletal agents for 7 h (33 mM nocodazole, 2 mM cytochalasin D, or 5 mM acrylamide) in DME, treated with 5 mM acrylamide for 7 h and then incubated in DME for 24 h (acrylamide reversal), and fixed, or treated with anti-CK19 antisense oligonucleotide for at least three passages. Centrosomes were counted in three-dimensional confocal deconvoluted images from colocalizations of CK19 or CK18 and g-tubulin as those shown in Fig. 2 and Fig. 3. The data from two independent experiments, counting 66 cells are shown as percent of centrosomes in the apical, deep cytoplasmic or lateral domains. For apical or lateral localization centrosomes at <0.3 mm of the corresponding domain of cortical CK19 signal were counted.

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