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Table I

Kinetics of Apoptosis Induced by Etoposide, Dexamethasone, Staurosporine, or Anti-CD95 Monoclonal Antibody (CH-11) in Jurkat and CEM-C7A T-leukemic Cell Lines

Time of treatmentJurkatCEM C7A
AO apoptosis
     
Control   0  1.2(±0.2)  0.3(±0.2) 
Etoposide   4       7(±1)       1(±1) 
  24      53(±6)      81(±13) 
Dexamethasone  24  ND       2(±3) 
  48  ND      50(±10) 
Staurosporine   4      13(±3)      37(±9) 
  24      48(±9)      79(±12) 
Anti-CD95 mAb  24      55(±3)      22(±6) 
1 ng/ml       
Time of treatmentJurkatCEM C7A
AO apoptosis
     
Control   0  1.2(±0.2)  0.3(±0.2) 
Etoposide   4       7(±1)       1(±1) 
  24      53(±6)      81(±13) 
Dexamethasone  24  ND       2(±3) 
  48  ND      50(±10) 
Staurosporine   4      13(±3)      37(±9) 
  24      48(±9)      79(±12) 
Anti-CD95 mAb  24      55(±3)      22(±6) 
1 ng/ml       

Percentage of apoptosis was assessed by fluorescence microscopy after acridine orange (AO) staining of cells. Results are expressed as mean(±SD) of three independent experiments.  

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