Effect of LAR Cross-linking on HeLa Cell Morphology
| Cross-linking antibody . | . | Fraction of spread cells with one or more long processes (%) . | ||||||
|---|---|---|---|---|---|---|---|---|
| Expt. 1 . | . | Expt. 2 . | . | Expt. 3 . | . | Expt. 4 . | ||
| None | 41.6% | 41.9% | 41.8% | 41.3% | ||||
| (331/795) | (131/313) | (76/182) | (57/138) | |||||
| Anti-LAR (75.3A) | 3.3% | 3.4% | 2.6% | 2.5% | ||||
| (21/630) | (6/176) | (5/190) | (2/81) | |||||
| Anti-β2 microglobulin | 41.7% | 36.7% | 53.8% | |||||
| (78/187) | (40/109) | (63/117) | ||||||
| Cross-linking antibody . | . | Fraction of spread cells with one or more long processes (%) . | ||||||
|---|---|---|---|---|---|---|---|---|
| Expt. 1 . | . | Expt. 2 . | . | Expt. 3 . | . | Expt. 4 . | ||
| None | 41.6% | 41.9% | 41.8% | 41.3% | ||||
| (331/795) | (131/313) | (76/182) | (57/138) | |||||
| Anti-LAR (75.3A) | 3.3% | 3.4% | 2.6% | 2.5% | ||||
| (21/630) | (6/176) | (5/190) | (2/81) | |||||
| Anti-β2 microglobulin | 41.7% | 36.7% | 53.8% | |||||
| (78/187) | (40/109) | (63/117) | ||||||
HeLa cells (1.2 × 105 cells/well) were plated on the laminin–nidogen complex. Antibody-induced cross-linking, cell fixation, and phase contrast microscopy were carried out as described in Fig. 5. In each experiment, fractions of spread cells with long extensions were quantified by counting cells from at least four randomly chosen fields. Expt., experiment.