Rockefeller University Press

Table III

Dynamic Parameters of Microtubules

	Wild type		Dynein mutant
	Rate	Number of events	Rate^*	Number of events
Total
Grow (μm/min)	0.491 ± 0.036	21	0.331^* ± 0.032	27
Shrink (μm/min)	1.350 ± 0.159	20	0.386^* ± 0.052	25
Catastrophe (s⁻¹)	0.006	15	0.003^‡	15
Rescue (s⁻¹)	0.002	2	0.002	7
Unbudded
Grow (μm/min)	0.514 ± 0.047	14	0.420 ± 0.074	7
Shrink (μm/min)	1.784 ± 0.190	11	0.620^* ± 0.148	6
Catastrophe (s⁻¹)	0.007	10	0.006	4
Rescue (s⁻¹)	−	−	0.002	1
Budded
Grow (μm/min)	0.446 ± 0.050	7	0.300^* ± 0.033	20
Shrink (μm/min)	0.820 ± 0.118	9	0.313^* ± 0.040	19
Catastrophe (s⁻¹)	0.005	5	0.003	11
Rescue (s⁻¹)	0.002	2	0.002	6

	Wild type		Dynein mutant
	Rate	Number of events	Rate^*	Number of events
Total
Grow (μm/min)	0.491 ± 0.036	21	0.331^* ± 0.032	27
Shrink (μm/min)	1.350 ± 0.159	20	0.386^* ± 0.052	25
Catastrophe (s⁻¹)	0.006	15	0.003^‡	15
Rescue (s⁻¹)	0.002	2	0.002	7
Unbudded
Grow (μm/min)	0.514 ± 0.047	14	0.420 ± 0.074	7
Shrink (μm/min)	1.784 ± 0.190	11	0.620^* ± 0.148	6
Catastrophe (s⁻¹)	0.007	10	0.006	4
Rescue (s⁻¹)	−	−	0.002	1
Budded
Grow (μm/min)	0.446 ± 0.050	7	0.300^* ± 0.033	20
Shrink (μm/min)	0.820 ± 0.118	9	0.313^* ± 0.040	19
Catastrophe (s⁻¹)	0.005	5	0.003	11
Rescue (s⁻¹)	0.002	2	0.002	6

A total of 27 and 29 microtubules were examined for wild-type and dynein-deficient cells, respectively. Rates of growing and shrinking are shown ± SEM.

Rates that are significantly different within a 95% confidence level ot those of wild type.

‡

Frequencies that are significantly different within a 90% confidence level to those of wild type.

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