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Table III

Dynamic Parameters of Microtubules

Wild typeDynein mutant
RateNumber of eventsRate*Number of events
Total         
 Grow (μm/min)  0.491 ± 0.036  21  0.331* ± 0.032  27 
 Shrink (μm/min)  1.350 ± 0.159  20  0.386* ± 0.052  25 
 Catastrophe (s−1 0.006  15  0.003  15 
 Rescue (s−1 0.002        2  0.002        7 
Unbudded         
 Grow (μm/min)  0.514 ± 0.047  14  0.420 ± 0.074        7 
 Shrink (μm/min)  1.784  ± 0.190  11  0.620* ± 0.148        6 
 Catastrophe (s−1 0.007  10  0.006        4 
 Rescue (s−1 −  −  0.002        1 
Budded         
 Grow (μm/min)  0.446 ± 0.050        7  0.300* ± 0.033  20 
 Shrink (μm/min)  0.820 ± 0.118         9  0.313* ± 0.040  19 
 Catastrophe (s−1 0.005        5  0.003  11 
 Rescue (s−1 0.002        2  0.002        6 
Wild typeDynein mutant
RateNumber of eventsRate*Number of events
Total         
 Grow (μm/min)  0.491 ± 0.036  21  0.331* ± 0.032  27 
 Shrink (μm/min)  1.350 ± 0.159  20  0.386* ± 0.052  25 
 Catastrophe (s−1 0.006  15  0.003  15 
 Rescue (s−1 0.002        2  0.002        7 
Unbudded         
 Grow (μm/min)  0.514 ± 0.047  14  0.420 ± 0.074        7 
 Shrink (μm/min)  1.784  ± 0.190  11  0.620* ± 0.148        6 
 Catastrophe (s−1 0.007  10  0.006        4 
 Rescue (s−1 −  −  0.002        1 
Budded         
 Grow (μm/min)  0.446 ± 0.050        7  0.300* ± 0.033  20 
 Shrink (μm/min)  0.820 ± 0.118         9  0.313* ± 0.040  19 
 Catastrophe (s−1 0.005        5  0.003  11 
 Rescue (s−1 0.002        2  0.002        6 

A total of 27 and 29 microtubules were examined for wild-type and dynein-deficient cells, respectively. Rates of growing and shrinking are shown ± SEM.  

*

 Rates that are significantly different within a 95% confidence level ot those of wild type.  

 Frequencies that are significantly different within a 90% confidence level to those of wild type.  

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