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Table II

Microtubule Interactions with the Cell Cortex

ParameterWild typeDynein mutant
Percentage of time at cortex (n = MTs)  42.7 (n = 39)  83.0 (n = 36) 
 Unbudded*  44.5 (n = 24)  92.8 (n = 10) 
 Budded  41.1 (n = 15)  79.3 (n = 26) 
Maximum length of MT (μm)   2.0   4.0 
 Unbudded   1.9   2.3 
 Budded   2.0   4.6 
Total cells analyzed  52  57 
 Unbudded  40  24 
 Budded  12  33 
 Total cortex interactions (%)  84.6  77.2 
  Unbudded  85.0  79.2 
  Budded  83.3  75.8 
  Hit cortex (%)  71.2  68.4 
   Unbudded  72.5  70.8 
   Budded  66.7  66.7 
  Grow at cortex (%)  32.7  19.3 
   Unbudded  35.0  16.7 
   Budded  25.0  21.2 
  Sweep on cortex (%)  32.7  33.3 
   Unbudded  25.0  33.3 
   Budded  58.3  33.3  
  Shrink at cortex (%)   5.8   − 
   Unbudded   2.5   − 
   Budded  16.7   − 
 Total nondynamic cells (%)   1.9  14.0 
  Unbudded   −  12.5 
  Budded   8.3  15.2 
ParameterWild typeDynein mutant
Percentage of time at cortex (n = MTs)  42.7 (n = 39)  83.0 (n = 36) 
 Unbudded*  44.5 (n = 24)  92.8 (n = 10) 
 Budded  41.1 (n = 15)  79.3 (n = 26) 
Maximum length of MT (μm)   2.0   4.0 
 Unbudded   1.9   2.3 
 Budded   2.0   4.6 
Total cells analyzed  52  57 
 Unbudded  40  24 
 Budded  12  33 
 Total cortex interactions (%)  84.6  77.2 
  Unbudded  85.0  79.2 
  Budded  83.3  75.8 
  Hit cortex (%)  71.2  68.4 
   Unbudded  72.5  70.8 
   Budded  66.7  66.7 
  Grow at cortex (%)  32.7  19.3 
   Unbudded  35.0  16.7 
   Budded  25.0  21.2 
  Sweep on cortex (%)  32.7  33.3 
   Unbudded  25.0  33.3 
   Budded  58.3  33.3  
  Shrink at cortex (%)   5.8   − 
   Unbudded   2.5   − 
   Budded  16.7   − 
 Total nondynamic cells (%)   1.9  14.0 
  Unbudded   −  12.5 
  Budded   8.3  15.2 
*

 Data from unbudded cells and cells containing a very small bud but no discernible spindle were combined for this and all subsequent measurements.  

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