Rockefeller University Press

Table IV

Percent of LAMP-1/LAMP-2 Point Mutant Chimera Expressed at the Cell Surface

Chimera	Cell line	n^*	Average surface expression (%)^‡
L/2b/2b S396T	pool	2	40
	3	3	44 ± 9
	6	3	45 ± 9

L/2b/2b R391Y	4	2	40
	5	2	31
	6	2	28
	8	2	36
	14	2	24
	17	2	32
	19	2	29
	23	2	44

L/2b/2b V397L	pool	3	8 ± 2
	7	3	4 ± 1
	13	3	6 ± 1

L2b/2b S396T, V397L	pool	6	8 ± 2
	6	3	5 ± 1
	19	2	6
	21	2	6
	24	3	5 ± 2

L/2c/2c L396V	pool	5	22 ± 2
	3	2	21
	12	2	28

Chimera	Cell line	n^*	Average surface expression (%)^‡
L/2b/2b S396T	pool	2	40
	3	3	44 ± 9
	6	3	45 ± 9

L/2b/2b R391Y	4	2	40
	5	2	31
	6	2	28
	8	2	36
	14	2	24
	17	2	32
	19	2	29
	23	2	44

L/2b/2b V397L	pool	3	8 ± 2
	7	3	4 ± 1
	13	3	6 ± 1

L2b/2b S396T, V397L	pool	6	8 ± 2
	6	3	5 ± 1
	19	2	6
	21	2	6
	24	3	5 ± 2

L/2c/2c L396V	pool	5	22 ± 2
	3	2	21
	12	2	28

n represents the number of independent experiments in which each sample was assayed in triplicate.

‡

average per cell line ± standard deviation; calculated from ¹²⁵I-labeled mAb-CV24 binding to intact and permeabilized cells as described in Materials and Methods.

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