Rockefeller University Press

Table II

Proteolytic Activity and Morphogenetic Behavior of End. Cells Lacking uPA, tPA, utPA, and PAI-1

Genotype	Cell lines	uPA^*	tPA^*	PAI-1^*	Behavior in fibrin gels^‡
wt+/+	b1, b3, e2, s2, m1, s1	+++	++	+	Variably large to small cysts and tubes (n = 39)
uPA−/−	u23, u24	−	+	++	Medium to small cysts and tubes (n = 18)
tPA−/−	t37, t38	++	−	++	Small cysts and tubes (n = 14)
uPA−/−, tPA−/−	ut1, ut2	−	−	+++	No cysts and no tubes; isolated cells and network of cell cords (n = 6)
PAI-1−/−	p1, p3	+++	+++	−	Variably large to small cysts and network of cell cords (n = 14)

Genotype	Cell lines	uPA^*	tPA^*	PAI-1^*	Behavior in fibrin gels^‡
wt+/+	b1, b3, e2, s2, m1, s1	+++	++	+	Variably large to small cysts and tubes (n = 39)
uPA−/−	u23, u24	−	+	++	Medium to small cysts and tubes (n = 18)
tPA−/−	t37, t38	++	−	++	Small cysts and tubes (n = 14)
uPA−/−, tPA−/−	ut1, ut2	−	−	+++	No cysts and no tubes; isolated cells and network of cell cords (n = 6)
PAI-1−/−	p1, p3	+++	+++	−	Variably large to small cysts and network of cell cords (n = 14)

Semiquantitative estimate determined by zymography (uPA, tPA) and reverse zymography (PAI-1).

‡

Cells grown in three-dimensional fibrin gels for 4–16 d in the absence of Trasylol; n = number of experiments. +++, high activity; ++, intermediate activity; +, low activity.

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