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Table 3.

VSM formation during micropexophagy in PpAtg18 AA and DD mutant strains

Straina VSM formationb 
 
 % % % % % 
Wt 55.5 (±5.14) 30.1 (±3.95) 10.7 (±1.54) 2.94 (±1.03) 0.178 (±0.182) 
AA 40.3 (±4.81) 36.7 (±2.84) 16.9 (±2.25) 4.11 (±1.02) 0.986 (±0.632) 
DD 45.1 (±6.45) 36.6 (±1.26) 13.6 (±4.27) 3.09 (±1.76) 0.260 (±0.261) 
Straina VSM formationb 
 
 % % % % % 
Wt 55.5 (±5.14) 30.1 (±3.95) 10.7 (±1.54) 2.94 (±1.03) 0.178 (±0.182) 
AA 40.3 (±4.81) 36.7 (±2.84) 16.9 (±2.25) 4.11 (±1.02) 0.986 (±0.632) 
DD 45.1 (±6.45) 36.6 (±1.26) 13.6 (±4.27) 3.09 (±1.76) 0.260 (±0.261) 

The difference in the average of VSMs per cell between PpAtg18Wt and PpATG18AA was significant (P < 0.05). Wt, wild type.

a

PpAtg18Wt, AA, or DD tagged 5×Flag were expressed under the PpATG18 promoter in Ppatg18Δ. Micropexophagy was induced by adapting cells from methanol to glucose medium for 30 min

b

VSM formation is indicated as the population of the cells (%) having the indicated number of vacuoles in a cell.

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