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Table 2.

Septation of vacuoles during hyperosmotic adaptation

Medium Straina The number of vacuoles/cellb 
  
  % % % % % 
SD Wt 68.6 (±5.76) 27.1 (±5.15) 1.74 (±1.43) 0 (±0) 0 (±0) 
 AA 45.6 (±10.6) 42.4 (±7.25) 6.44 (±2.43) 1.06 (±0.495) 0.292 (±0.413) 
 DD 57.0 (±5.62) 36.5 (±5.50) 3.65 (±0.943) 0.960 (±0.698) 0.273 (±0.387) 
SD + 0.9 M NaCl Wt 27.9 (±9.90) 42.7 (±4.63) 20.6 (±5.25) 5.60 (±2.72) 2.32 (±1.33) 
 AA 17.4 (±2.93) 42.1 (±7.15) 25.4 (±2.58) 9.56 (±3.26) 4.73 (±4.95) 
 DD 35.2 (±9.76) 39.5 (±3.80) 17.6 (±3.82) 4.68 (±1.60) 1.60 (±1.71) 
Medium Straina The number of vacuoles/cellb 
  
  % % % % % 
SD Wt 68.6 (±5.76) 27.1 (±5.15) 1.74 (±1.43) 0 (±0) 0 (±0) 
 AA 45.6 (±10.6) 42.4 (±7.25) 6.44 (±2.43) 1.06 (±0.495) 0.292 (±0.413) 
 DD 57.0 (±5.62) 36.5 (±5.50) 3.65 (±0.943) 0.960 (±0.698) 0.273 (±0.387) 
SD + 0.9 M NaCl Wt 27.9 (±9.90) 42.7 (±4.63) 20.6 (±5.25) 5.60 (±2.72) 2.32 (±1.33) 
 AA 17.4 (±2.93) 42.1 (±7.15) 25.4 (±2.58) 9.56 (±3.26) 4.73 (±4.95) 
 DD 35.2 (±9.76) 39.5 (±3.80) 17.6 (±3.82) 4.68 (±1.60) 1.60 (±1.71) 

The difference in the average of total vacuoles per cell between PpAtg18Wt and PpATG18AA was marginally significant (P < 0.1). Wt, wild type.

a

PpAtg18Wt, AA, or DD tagged 5×Flag were expressed under the PpATG18 promoter in Ppatg18Δ. Cells were grown in SD medium, and shifted to SD or SD + 0.9 M NaCl for 1 h.

b

Vacuolar fission indicated as the population of the cells (%) having the indicated number of vacuoles in a cell.

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