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Table 4.
Whole-cell current amplitudes in EDTA for wild-type GluN1/GluN2A or tryptophan-substituted GluN1 or GluN2A NMDARs where whole-cell currents were significantly reduced
ConstructIpeakIsteady-statenEstimated channel number (N)
 pA pA   
N1/N2A −1,170 ± 70 −760 ± 50 13 220 ± 20 
N1(N812W) −350 ± 40* −96 ± 12* 1,340 ± 390 
N1(M813W) −590 ± 90 −220 ± 30* 1,810 ± 340 
N1(V816W) −120 ± 20* −80 ± 12* 920 ± 210 
N1(G827W) −150 ± 30* −54 ± 13* 34 ± 7 
N1(E834W) −270 ± 40* −150 ± 30* 48 ± 7 
N2A(M817W) −35 ± 4* −27 ± 3* 3,830 ± 330 
N2A(G819W) −26 ± 6* −5 ± 1* 280 ± 60 
N2A(E838W) −420 ± 50* −230 ± 30* 33 ± 5 
ConstructIpeakIsteady-statenEstimated channel number (N)
 pA pA   
N1/N2A −1,170 ± 70 −760 ± 50 13 220 ± 20 
N1(N812W) −350 ± 40* −96 ± 12* 1,340 ± 390 
N1(M813W) −590 ± 90 −220 ± 30* 1,810 ± 340 
N1(V816W) −120 ± 20* −80 ± 12* 920 ± 210 
N1(G827W) −150 ± 30* −54 ± 13* 34 ± 7 
N1(E834W) −270 ± 40* −150 ± 30* 48 ± 7 
N2A(M817W) −35 ± 4* −27 ± 3* 3,830 ± 330 
N2A(G819W) −26 ± 6* −5 ± 1* 280 ± 60 
N2A(E838W) −420 ± 50* −230 ± 30* 33 ± 5 

Values shown are mean ± SEM. Tagged values are significantly less (*) or greater (^) than wild type (P < 0.05, t test). n indicates the number of whole-cell recordings. Currents were recorded in the whole-cell mode at a holding potential of −70 mV, as in Table 1, but with our standard extracellular solution (pH 7.2) also containing 0.01 mM EDTA. These conditions were aligned to those for on-cell single-channel recording except for the pH. The estimated channel number mediating the observed steady-state whole-cell current amplitudes (Isteady-state) was derived from: Isteady-state = N * Po * i, where N is the approximate number of channels on the membrane, Po is the equilibrium Popen, and i is single-channel current amplitude corrected for difference in membrane potential assuming Ohmic behavior (Table 3).

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