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Table 3.
Single-channel properties of wild-type GluN1/GluN2A or tryptophan-substituted GluN1 or GluN2A NMDARs where whole-cell currents were significantly changed
ConstructTotal events (# of patches)ieq. PoMCTMOT
  pA  ms ms 
N1/N2A 1,531,680 (10) −7.2 ± 0.1 0.69 ± 0.04 2.3 ± 0.2 5.5 ± 0.5 
N1(N812W) 147,374 (5) −4.1 ± 0.5* 0.03 ± 0.01* 31.8 ± 10.3* 0.40 ± 0.07* 
N1(M813W) 484,988 (4) −4.4 ± 0.4* 0.04 ± 0.01* 7.3 ± 1.5* 0.28 ± 0.05* 
N1(V816W) 475,752 (4) −5.3 ± 0.7* 0.02 ± 0.01* 7.2 ± 2.2 0.13 ± 0.01* 
N1(G827W) 218,498 (4) −7.6 ± 0.5 0.30 ± 0.05* 11.6 ± 1.9* 4.7 ± 0.8 
N1(E834W) 198,394 (4) −7.0 ± 0.3 0.64 ± 0.07 3.8 ± 0.8 6.5 ± 0.6 
N2A(M817W) 20,985 (5) −8.0 ± 0.2 0.001 ± 0.0005* 213 ± 73* 0.20 ± 0.10* 
N2A(G819W) 27,892 (5) −5.4 ± 0.3* 0.005 ± 0.001* 73.8 ± 7.1* 0.33 ± 0.03* 
N2A(E838W) 81,550 (4) −6.6 ± 0.4 0.54 ± 0.08 9.1 ± 3.4 8.8 ± 0.6* 
ConstructTotal events (# of patches)ieq. PoMCTMOT
  pA  ms ms 
N1/N2A 1,531,680 (10) −7.2 ± 0.1 0.69 ± 0.04 2.3 ± 0.2 5.5 ± 0.5 
N1(N812W) 147,374 (5) −4.1 ± 0.5* 0.03 ± 0.01* 31.8 ± 10.3* 0.40 ± 0.07* 
N1(M813W) 484,988 (4) −4.4 ± 0.4* 0.04 ± 0.01* 7.3 ± 1.5* 0.28 ± 0.05* 
N1(V816W) 475,752 (4) −5.3 ± 0.7* 0.02 ± 0.01* 7.2 ± 2.2 0.13 ± 0.01* 
N1(G827W) 218,498 (4) −7.6 ± 0.5 0.30 ± 0.05* 11.6 ± 1.9* 4.7 ± 0.8 
N1(E834W) 198,394 (4) −7.0 ± 0.3 0.64 ± 0.07 3.8 ± 0.8 6.5 ± 0.6 
N2A(M817W) 20,985 (5) −8.0 ± 0.2 0.001 ± 0.0005* 213 ± 73* 0.20 ± 0.10* 
N2A(G819W) 27,892 (5) −5.4 ± 0.3* 0.005 ± 0.001* 73.8 ± 7.1* 0.33 ± 0.03* 
N2A(E838W) 81,550 (4) −6.6 ± 0.4 0.54 ± 0.08 9.1 ± 3.4 8.8 ± 0.6* 

Values shown are mean ± SEM for single-channel current amplitude (i), equilibrium open probability (eq. Po), MCT, and MOT. Single-channel currents were recorded in the on-cell mode at approximately −100 mV and analyzed in QuB (see Materials and methods). Number of patches is in parenthesis to the right of total events. Eq. Po is the fractional occupancy of the open states in the entire single-channel recording, including long-lived closed states. All data were idealized and fit at a dead time of 40 µs. Asterisks indicate values significantly different from wild type (P < 0.05, t test).

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