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Table 1.
Raw macroscopic peak current amplitudes, shown as normalized values in Fig. 3 B, for wild-type GluN1/GluN2A or GluN1/GluN2A containing tryptophan substitutions in either GluN1 or GluN2A M4 segments
Tryptophans in GluN1 subunitTryptophans in GluN2A subunit
ConstructIpeaknConstructIpeakn
 pA   pA  
N1/N2A −750 ± 50 18    
F810W −830 ± 290 I814W −680 ± 125 
E811W −720 ± 95 D815W −580 ± 110 
N812W −28 ± 6* N816W ND 
M813W −65 ± 9* M817W −17 ± 3* 
A814W −530 ± 130 A818W −1,160 ± 175 
G815W −330 ± 90 G819W −11 ± 2* 
V816W −250 ± 30* V820W ND 
F817W −1,150 ± 240 F821W −370 ± 35 
M818W −355 ± 110 Y822W −1,500 ± 120 
L819W −2,010 ± 210^ M823W −2,090 ± 100^ 
V820W −1,830 ± 280 L824W −1,030 ± 150 
A821W −760 ± 110 A825W −860 ± 120 
G822W −1,050 ± 220 A826W −560 ± 65 
G823W −730 ± 110 A827W −1,680 ± 205 
I824W −730 ± 150 M828W −1,110 ± 150 
V825W −330 ± 20 A829W −890 ± 130 
A826W −880 ± 170 L830W −1,830 ± 470 
G827W −78 ± 8* S831W ND 
I828W −720 ± 90 L832W −760 ± 90 
F829W −960 ± 130 I833W −1,340 ± 100 
L830W −410 ± 45 T834W −1,000 ± 160 
I831W −670 ± 60 F835W −1,020 ± 190 
F832W −1,290 ± 220 I836W −1,320 ± 160 
I833W −1,110 ± 130 W837 −750 ± 50 18 
E834W −100 ± 10* E838W −265 ± 40* 
Tryptophans in GluN1 subunitTryptophans in GluN2A subunit
ConstructIpeaknConstructIpeakn
 pA   pA  
N1/N2A −750 ± 50 18    
F810W −830 ± 290 I814W −680 ± 125 
E811W −720 ± 95 D815W −580 ± 110 
N812W −28 ± 6* N816W ND 
M813W −65 ± 9* M817W −17 ± 3* 
A814W −530 ± 130 A818W −1,160 ± 175 
G815W −330 ± 90 G819W −11 ± 2* 
V816W −250 ± 30* V820W ND 
F817W −1,150 ± 240 F821W −370 ± 35 
M818W −355 ± 110 Y822W −1,500 ± 120 
L819W −2,010 ± 210^ M823W −2,090 ± 100^ 
V820W −1,830 ± 280 L824W −1,030 ± 150 
A821W −760 ± 110 A825W −860 ± 120 
G822W −1,050 ± 220 A826W −560 ± 65 
G823W −730 ± 110 A827W −1,680 ± 205 
I824W −730 ± 150 M828W −1,110 ± 150 
V825W −330 ± 20 A829W −890 ± 130 
A826W −880 ± 170 L830W −1,830 ± 470 
G827W −78 ± 8* S831W ND 
I828W −720 ± 90 L832W −760 ± 90 
F829W −960 ± 130 I833W −1,340 ± 100 
L830W −410 ± 45 T834W −1,000 ± 160 
I831W −670 ± 60 F835W −1,020 ± 190 
F832W −1,290 ± 220 I836W −1,320 ± 160 
I833W −1,110 ± 130 W837 −750 ± 50 18 
E834W −100 ± 10* E838W −265 ± 40* 

Values shown are mean ± SEM. n indicates the number of whole-cell recordings. Peak current amplitudes were recorded in the whole-cell mode at −70 mV (e.g., Fig. 3 A). GluN1 constructs were co-expressed with wild-type GluN2A and vice-versa. Tagged values are significantly less (*) or greater (^) than wild-type (P < 0.05, two-tailed Student’s t test, unpaired). ND, no glutamate-activated currents detected. As done previously (Salussolia et al., 2011), constructs were tested on at least two different transfection cycles with wild type tested every other transfection cycle. Constructs that showed no detectable glutamate-activated current were tested on at least one additional transfection cycle.

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