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Table 1.
Fitted parameters from concentration–response experiments
ConstructVoltagelog EC50(1)EC50(1)h1log EC50(2)EC50(2)h2ImaxFrac
 mV  µM   µM    
WT 80 −6.50 (−6.52 to −6.47) 0.316 1.88 (1.69 to 2.07) −3.38 (−3.85 to −2.91) 417 0.665 (0.341 to 0.989) 1.46 (1.29 to 1.64) 0.666 (0.566 to 0.766) 
 −80 −6.09 (−6.11 to −6.06) 0.813 2.23 (1.98 to 2.47) −3.38 (−3.85 to −2.91) 417 0.665 (0.341 to 0.989) 1.46 (1.29 to 1.64) 0.666 (0.566 to 0.766) 
E702Q 80 −4.41 (−4.46 to −4.36) 38.8 1.09 (0.963 to 1.21)    1.03 (0.996 to 1.06)  
 −80 −3.78 (−3.81 to −3.74) 167 1.19 (1.10 to 1.28)    1.13 (1.10 to 1.16)  
K588E/E702Q 80 −5.03 (−5.08 to −4.97) 9.44 1.01 (0.910 to 1.11)    1.02 (0.987 to 1.06)  
 −80 −4.44 (−4.51 to −4.38) 36 1.07 (0.963 to 1.18)    1.11 (1.05 to 1.17)  
WT_WT 80 −6.68 (−6.71 to −6.65) 0.209 1.60 (1.39 to 1.81) −3.14 (−3.97 to −2.31) 724 0.511 (0.196 to 0.826) 1.57 (1.24 to 1.89) 0.609 (0.446 to 0.772) 
 −80 −6.23 (−6.26 to −6.20) 0.589 1.78 (1.57 to 1.98) −3.14 (−3.97 to −2.31) 724 0.511 (0.196 to 0.826) 1.57 (1.24 to 1.89) 0.609 (0.446 to 0.772) 
WT_E654Q 80 −6.52 (−6.59 to −6.44) 0.302 1.48 (1.18 to 1.77) −3.76 (−4.03 to −3.49) 174 0.765 (0.304 to 1.22) 1.46 (1.35 to 1.57) 0.653 (0.546 to 0.760) 
 −80 −6.11 (−6.16 to −6.05) 0.772 1.76 (1.43 to 2.08) −3.76 (−4.03 to −3.49) 174 0.765 (0.304 to 1.22) 1.46 (1.35 to 1.57) 0.653 (0.546 to 0.760) 
WT_E702Q (biphasic; no constraints) 80 −6.28 (−6.33 to −6.22) 0.525 1.78 (1.45 to 2.12) −4.12 (−4.18 to −4.06) 75.9 0.851 (0.754 to 0.948) 1.00 (0.978 to 1.02) 0.312 (0.284 to 0.340) 
 −80 −5.89 (−5.95 to −5.83) 1.29 1.96 (1.61 to 2.31) −3.72 (−3.76 to −3.68) 191 1.21 (1.06 to 1.37) 1.00 (0.978 to 1.02) 0.312 (0.284 to 0.340) 
WT_E702Q (triphasic; A = 0.6, log EC50(3) = −3.14, h3 = 0.511, h1 = 1.88, h2 = 1.09) 80 −6.26 (−6.31 to −6.21) 0.548 =1.88 −4.21 (−4.29 to −4.13) 61.8 =1.09 1.25 (1.22 to 1.27) 0.517 (0.495 to 0.538) 
 −80 −5.92 (−5.97 to −5.87) 1.20 =2.23 −3.77 (−3.82 to −3.72) 171 =1.19 1.41 (1.38 to 1.44) 0.444 (0.424 to 0.463) 
WT_E702Q (triphasic; A = 0.6, log EC50(3) = −3.14, h3 = 0.511) 80 −6.29 (−6.34 to −6.23) 0.514 1.88 (1.52 to 2.24) −4.24 (−4.30 to −4.17) 58 0.921 (0.795 to 1.05) 1.06 (1.04 to 1.07) 0.477 (0.442 to 0.513) 
 −80 −5.88 (−5.94 to −5.82) 1.32 1.99 (1.63 to 2.36) −3.75 (−3.79 to −3.70) 179 1.36 (1.15 to 1.57) 1.06 (1.04 to 1.07) 0.477 (0.442 to 0.513) 
K588E_E702Q (biphasic; log EC50(1) = −6.46, h1 = 1.42) 80 = −6.46 =0.347 =1.42 −4.28 (−4.34 to −4.21) 52.5 1.13 (1.01 to 1.24) 1.16 (1.10 to 1.22) 0.015 (0.0055 to 0.024) 
 −80    −3.79 (−3.80 to −3.77) 163.2 1.49 (1.44 to 1.55) 1.40 (1.39 to 1.42)  
WT_K588E/E702Q (biphasic) 80 −6.29 (−6.41 to −6.17) 0.513 1.56 (0.811 to 2.30) −4.20 (−4.61 to −3.79) 63.1 0.616 (0.229 to 1.00) 1.20 (1.08 to 1.31) 0.412 (0.176 to 0.648) 
 −80 −5.97 (−6.05 to −5.88) 1.07 1.82 (1.39 to 2.24) −3.82 (−4.03 to −3.61) 151 1.11 (0.505 to 1.71) 1.15 (1.08 to 1.22) 0.599 (0.506 to 0.692) 
ConstructVoltagelog EC50(1)EC50(1)h1log EC50(2)EC50(2)h2ImaxFrac
 mV  µM   µM    
WT 80 −6.50 (−6.52 to −6.47) 0.316 1.88 (1.69 to 2.07) −3.38 (−3.85 to −2.91) 417 0.665 (0.341 to 0.989) 1.46 (1.29 to 1.64) 0.666 (0.566 to 0.766) 
 −80 −6.09 (−6.11 to −6.06) 0.813 2.23 (1.98 to 2.47) −3.38 (−3.85 to −2.91) 417 0.665 (0.341 to 0.989) 1.46 (1.29 to 1.64) 0.666 (0.566 to 0.766) 
E702Q 80 −4.41 (−4.46 to −4.36) 38.8 1.09 (0.963 to 1.21)    1.03 (0.996 to 1.06)  
 −80 −3.78 (−3.81 to −3.74) 167 1.19 (1.10 to 1.28)    1.13 (1.10 to 1.16)  
K588E/E702Q 80 −5.03 (−5.08 to −4.97) 9.44 1.01 (0.910 to 1.11)    1.02 (0.987 to 1.06)  
 −80 −4.44 (−4.51 to −4.38) 36 1.07 (0.963 to 1.18)    1.11 (1.05 to 1.17)  
WT_WT 80 −6.68 (−6.71 to −6.65) 0.209 1.60 (1.39 to 1.81) −3.14 (−3.97 to −2.31) 724 0.511 (0.196 to 0.826) 1.57 (1.24 to 1.89) 0.609 (0.446 to 0.772) 
 −80 −6.23 (−6.26 to −6.20) 0.589 1.78 (1.57 to 1.98) −3.14 (−3.97 to −2.31) 724 0.511 (0.196 to 0.826) 1.57 (1.24 to 1.89) 0.609 (0.446 to 0.772) 
WT_E654Q 80 −6.52 (−6.59 to −6.44) 0.302 1.48 (1.18 to 1.77) −3.76 (−4.03 to −3.49) 174 0.765 (0.304 to 1.22) 1.46 (1.35 to 1.57) 0.653 (0.546 to 0.760) 
 −80 −6.11 (−6.16 to −6.05) 0.772 1.76 (1.43 to 2.08) −3.76 (−4.03 to −3.49) 174 0.765 (0.304 to 1.22) 1.46 (1.35 to 1.57) 0.653 (0.546 to 0.760) 
WT_E702Q (biphasic; no constraints) 80 −6.28 (−6.33 to −6.22) 0.525 1.78 (1.45 to 2.12) −4.12 (−4.18 to −4.06) 75.9 0.851 (0.754 to 0.948) 1.00 (0.978 to 1.02) 0.312 (0.284 to 0.340) 
 −80 −5.89 (−5.95 to −5.83) 1.29 1.96 (1.61 to 2.31) −3.72 (−3.76 to −3.68) 191 1.21 (1.06 to 1.37) 1.00 (0.978 to 1.02) 0.312 (0.284 to 0.340) 
WT_E702Q (triphasic; A = 0.6, log EC50(3) = −3.14, h3 = 0.511, h1 = 1.88, h2 = 1.09) 80 −6.26 (−6.31 to −6.21) 0.548 =1.88 −4.21 (−4.29 to −4.13) 61.8 =1.09 1.25 (1.22 to 1.27) 0.517 (0.495 to 0.538) 
 −80 −5.92 (−5.97 to −5.87) 1.20 =2.23 −3.77 (−3.82 to −3.72) 171 =1.19 1.41 (1.38 to 1.44) 0.444 (0.424 to 0.463) 
WT_E702Q (triphasic; A = 0.6, log EC50(3) = −3.14, h3 = 0.511) 80 −6.29 (−6.34 to −6.23) 0.514 1.88 (1.52 to 2.24) −4.24 (−4.30 to −4.17) 58 0.921 (0.795 to 1.05) 1.06 (1.04 to 1.07) 0.477 (0.442 to 0.513) 
 −80 −5.88 (−5.94 to −5.82) 1.32 1.99 (1.63 to 2.36) −3.75 (−3.79 to −3.70) 179 1.36 (1.15 to 1.57) 1.06 (1.04 to 1.07) 0.477 (0.442 to 0.513) 
K588E_E702Q (biphasic; log EC50(1) = −6.46, h1 = 1.42) 80 = −6.46 =0.347 =1.42 −4.28 (−4.34 to −4.21) 52.5 1.13 (1.01 to 1.24) 1.16 (1.10 to 1.22) 0.015 (0.0055 to 0.024) 
 −80    −3.79 (−3.80 to −3.77) 163.2 1.49 (1.44 to 1.55) 1.40 (1.39 to 1.42)  
WT_K588E/E702Q (biphasic) 80 −6.29 (−6.41 to −6.17) 0.513 1.56 (0.811 to 2.30) −4.20 (−4.61 to −3.79) 63.1 0.616 (0.229 to 1.00) 1.20 (1.08 to 1.31) 0.412 (0.176 to 0.648) 
 −80 −5.97 (−6.05 to −5.88) 1.07 1.82 (1.39 to 2.24) −3.82 (−4.03 to −3.61) 151 1.11 (0.505 to 1.71) 1.15 (1.08 to 1.22) 0.599 (0.506 to 0.692) 

Asymptotic 95% confidence intervals are in parentheses; values preceded by = were constrained during fitting; values that are identical at 80 and −80 mV were constrained to a shared but freely varying value; data at 80 and −80 mV were normalized to their respective Imax values before global fitting.

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