Comparison of tsA201 experiments and simulations
Property | Experiment | Model |
Pipette resistance (MΩ) | 3–5 | 4.4 |
Plasma membrane area (pF//µm2) | 20//2,000 | 8.0//804 |
Diffusion coefficient melatonin (cm2/s) | 6 × 10−6a | 6 × 10−6 |
Cell-bath equilibration time constant for melatonin (s) | 3.5 | 3.54 |
Membrane permeability melatonin (µm/s) | - | 1.7 |
Exponential melatonin space constant outside cell (µm) | 30 | 22 |
Extracellular concentration with 10 mM melatonin in the whole-cell pipette (µM) | 2.8 | 11.6 |
Extracellular concentration with 10 mM melatonin in the on-cell pipette (µM) | 0.56 | 2.3 |
Property | Experiment | Model |
Pipette resistance (MΩ) | 3–5 | 4.4 |
Plasma membrane area (pF//µm2) | 20//2,000 | 8.0//804 |
Diffusion coefficient melatonin (cm2/s) | 6 × 10−6a | 6 × 10−6 |
Cell-bath equilibration time constant for melatonin (s) | 3.5 | 3.54 |
Membrane permeability melatonin (µm/s) | - | 1.7 |
Exponential melatonin space constant outside cell (µm) | 30 | 22 |
Extracellular concentration with 10 mM melatonin in the whole-cell pipette (µM) | 2.8 | 11.6 |
Extracellular concentration with 10 mM melatonin in the on-cell pipette (µM) | 0.56 | 2.3 |
Rice et al. (1985).