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Table 2.

Determination of concentrations of total zinc in rat skeletal muscle tissues using TPEN in combination with the BAPTA method

Muscle type Simple subtraction [ZnT]WM Corrected simple subtraction [ZnT]WM,corrected 
 mmole/kga mmole/kga 
Soleusb 0.319 ± 0.046 0.370 ± 0.054 
Plantarisb 0.152 ± 0.031 0.176 ± 0.036 
Muscle type Simple subtraction [ZnT]WM Corrected simple subtraction [ZnT]WM,corrected 
 mmole/kga mmole/kga 
Soleusb 0.319 ± 0.046 0.370 ± 0.054 
Plantarisb 0.152 ± 0.031 0.176 ± 0.036 

The normalized concentrations of total zinc ([ZnT]WM or [ZnT]WM,corrected; mmole/kg) estimated with or without correction using either soleus or plantaris muscle tissues are summarized here. Mean ± SEM. Two-way ANOVA was conducted on four groups of concentration of total zinc values using the simple subtraction or corrected simple subtraction, in either soleus or plantaris to determine which variable—the correction made on the simple subtraction (variable 1) and/or muscle types (variable 2)—was significant.

a

The correction calculation (variable 1) was found to be significant (P < 0.01).

b

The muscle type (variable 2) was also found to be significant (P < 0.05); n = 3 for each muscle type.

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