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Table 1.

SteT random mutants studied in this paper

Mutant TMD 
L247M 
L63R 
L52Q 
A305T/T410S 8–12 
F49Y/L297V 2–8 
I243P/A383S 7–11 
P226Q 
M392V 11 
I235V 
F89S/A105T 3–3 
I235F 
G61D/L78V 2–3 
G62C/F304S 2–8 
I285V 
L199P 
A197V/G232D 6–7 
G152D/V370M 1-9 
G69D 
G87D 
F49Y 
F16L/I99V 1–3 
R374C 10 
T159I/S298T 5–8 
P34Q 
I132F 
F371I/C415Y 10–12 
V154E/G161S 5–5 
L247V 
G215D 
G23R 
A136E 
G103S/L279P 3–8 
A369G 
G35R/G55D 1–2 
L199M/L417M 6–12 
A196T 
C141W 
E67K/E308K 2–8 
F203S/R376P 6–10 
F139L 
G123D/I164T 3–5 
I107F/H249Y 3–7 
G54S 
F49Y/N347Y 2–9 
I336N/M413S 9–12 
F402S 11 
A60E/C168R 2–5 
A267V 
M32V/M342L 1–9 
A424T 12 
A109P 
A398P 11 
G283V 
F31I 
G27A/T156S 1–5 
R374H 10 
L53P 
F391Y 11 
W51R/L338Q 2–9 
L14Q/T230A 1–7 
F31L 
A39F 
A339D 11 
L210Q/M229V 6–7 
N193D 
G161N 
C291S 
S303T 
I134V/A377T 4–10 
C168Y/L233M 5–7 
Mutants were generated by error-prone PCR according the protocol described in Materials and methods. The TMD column indicates the number of TMDs where the substitutions are located. Mutants are ordered as in Fig. 3, on the basis of their relative expression yield with respect to WT. 
Mutant TMD 
L247M 
L63R 
L52Q 
A305T/T410S 8–12 
F49Y/L297V 2–8 
I243P/A383S 7–11 
P226Q 
M392V 11 
I235V 
F89S/A105T 3–3 
I235F 
G61D/L78V 2–3 
G62C/F304S 2–8 
I285V 
L199P 
A197V/G232D 6–7 
G152D/V370M 1-9 
G69D 
G87D 
F49Y 
F16L/I99V 1–3 
R374C 10 
T159I/S298T 5–8 
P34Q 
I132F 
F371I/C415Y 10–12 
V154E/G161S 5–5 
L247V 
G215D 
G23R 
A136E 
G103S/L279P 3–8 
A369G 
G35R/G55D 1–2 
L199M/L417M 6–12 
A196T 
C141W 
E67K/E308K 2–8 
F203S/R376P 6–10 
F139L 
G123D/I164T 3–5 
I107F/H249Y 3–7 
G54S 
F49Y/N347Y 2–9 
I336N/M413S 9–12 
F402S 11 
A60E/C168R 2–5 
A267V 
M32V/M342L 1–9 
A424T 12 
A109P 
A398P 11 
G283V 
F31I 
G27A/T156S 1–5 
R374H 10 
L53P 
F391Y 11 
W51R/L338Q 2–9 
L14Q/T230A 1–7 
F31L 
A39F 
A339D 11 
L210Q/M229V 6–7 
N193D 
G161N 
C291S 
S303T 
I134V/A377T 4–10 
C168Y/L233M 5–7 
Mutants were generated by error-prone PCR according the protocol described in Materials and methods. The TMD column indicates the number of TMDs where the substitutions are located. Mutants are ordered as in Fig. 3, on the basis of their relative expression yield with respect to WT. 
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