SteT random mutants studied in this paper
| Mutant | TMD |
| L247M | 7 |
| L63R | 2 |
| L52Q | 2 |
| A305T/T410S | 8–12 |
| F49Y/L297V | 2–8 |
| I243P/A383S | 7–11 |
| P226Q | 7 |
| M392V | 11 |
| I235V | 7 |
| F89S/A105T | 3–3 |
| I235F | 7 |
| G61D/L78V | 2–3 |
| G62C/F304S | 2–8 |
| I285V | 8 |
| L199P | 6 |
| A197V/G232D | 6–7 |
| G152D/V370M | 1-9 |
| G69D | 2 |
| G87D | 3 |
| F49Y | 2 |
| F16L/I99V | 1–3 |
| R374C | 10 |
| T159I/S298T | 5–8 |
| P34Q | 1 |
| I132F | 4 |
| F371I/C415Y | 10–12 |
| V154E/G161S | 5–5 |
| L247V | 7 |
| G215D | 6 |
| G23R | 1 |
| A136E | 4 |
| G103S/L279P | 3–8 |
| A369G | 9 |
| G35R/G55D | 1–2 |
| L199M/L417M | 6–12 |
| A196T | 6 |
| C141W | 4 |
| E67K/E308K | 2–8 |
| F203S/R376P | 6–10 |
| F139L | 4 |
| G123D/I164T | 3–5 |
| I107F/H249Y | 3–7 |
| G54S | 2 |
| F49Y/N347Y | 2–9 |
| I336N/M413S | 9–12 |
| F402S | 11 |
| A60E/C168R | 2–5 |
| A267V | 8 |
| M32V/M342L | 1–9 |
| A424T | 12 |
| A109P | 3 |
| A398P | 11 |
| G283V | 8 |
| F31I | 1 |
| G27A/T156S | 1–5 |
| R374H | 10 |
| L53P | 2 |
| F391Y | 11 |
| W51R/L338Q | 2–9 |
| L14Q/T230A | 1–7 |
| F31L | 1 |
| A39F | 1 |
| A339D | 11 |
| L210Q/M229V | 6–7 |
| N193D | 6 |
| G161N | 5 |
| C291S | 8 |
| S303T | 8 |
| I134V/A377T | 4–10 |
| C168Y/L233M | 5–7 |
| Mutants were generated by error-prone PCR according the protocol described in Materials and methods. The TMD column indicates the number of TMDs where the substitutions are located. Mutants are ordered as in Fig. 3, on the basis of their relative expression yield with respect to WT. | |
| Mutant | TMD |
| L247M | 7 |
| L63R | 2 |
| L52Q | 2 |
| A305T/T410S | 8–12 |
| F49Y/L297V | 2–8 |
| I243P/A383S | 7–11 |
| P226Q | 7 |
| M392V | 11 |
| I235V | 7 |
| F89S/A105T | 3–3 |
| I235F | 7 |
| G61D/L78V | 2–3 |
| G62C/F304S | 2–8 |
| I285V | 8 |
| L199P | 6 |
| A197V/G232D | 6–7 |
| G152D/V370M | 1-9 |
| G69D | 2 |
| G87D | 3 |
| F49Y | 2 |
| F16L/I99V | 1–3 |
| R374C | 10 |
| T159I/S298T | 5–8 |
| P34Q | 1 |
| I132F | 4 |
| F371I/C415Y | 10–12 |
| V154E/G161S | 5–5 |
| L247V | 7 |
| G215D | 6 |
| G23R | 1 |
| A136E | 4 |
| G103S/L279P | 3–8 |
| A369G | 9 |
| G35R/G55D | 1–2 |
| L199M/L417M | 6–12 |
| A196T | 6 |
| C141W | 4 |
| E67K/E308K | 2–8 |
| F203S/R376P | 6–10 |
| F139L | 4 |
| G123D/I164T | 3–5 |
| I107F/H249Y | 3–7 |
| G54S | 2 |
| F49Y/N347Y | 2–9 |
| I336N/M413S | 9–12 |
| F402S | 11 |
| A60E/C168R | 2–5 |
| A267V | 8 |
| M32V/M342L | 1–9 |
| A424T | 12 |
| A109P | 3 |
| A398P | 11 |
| G283V | 8 |
| F31I | 1 |
| G27A/T156S | 1–5 |
| R374H | 10 |
| L53P | 2 |
| F391Y | 11 |
| W51R/L338Q | 2–9 |
| L14Q/T230A | 1–7 |
| F31L | 1 |
| A39F | 1 |
| A339D | 11 |
| L210Q/M229V | 6–7 |
| N193D | 6 |
| G161N | 5 |
| C291S | 8 |
| S303T | 8 |
| I134V/A377T | 4–10 |
| C168Y/L233M | 5–7 |
| Mutants were generated by error-prone PCR according the protocol described in Materials and methods. The TMD column indicates the number of TMDs where the substitutions are located. Mutants are ordered as in Fig. 3, on the basis of their relative expression yield with respect to WT. | |