Rockefeller University Press

Table 1.

Summary of the results on HCN channel conductance

HCN channels	Experimental method	Results (pS)
HCN channels	Experimental method	Pmax = 1 (±SD)	Pmax from NSNA (±SD)
mHCN1-EGFP	PCF	0.46 ± 0.07
mHCN2-EGFP	PCF	1.46 ± 0.26	1.71 ± 0.34
spHCN-EGFP	PCF	0.88 ± 0.22	1.13 ± 0.29
		Macroscopic current (activation; ±SE)	Tail current (deactivation; ±SE)
mHCN2-EGFP	NSNA	2.70 ± 0.21 (n = 3)	2.29 ± 0.61 (n = 3)
mHCN2	NSNA	2.37 ± 0.24 (n = 4)	2.27 ± 0.28 (n = 4)
spHCN-EGFP	NSNA	1.03 ± 0.11 (n = 3)	0.99 ± 0.27 (n = 3)
spHCN	NSNA	0.76 ± 0.16 (n = 4)	0.49 ± 0.10 (n = 4)

HCN channels	Experimental method	Results (pS)
HCN channels	Experimental method	Pmax = 1 (±SD)	Pmax from NSNA (±SD)
mHCN1-EGFP	PCF	0.46 ± 0.07
mHCN2-EGFP	PCF	1.46 ± 0.26	1.71 ± 0.34
spHCN-EGFP	PCF	0.88 ± 0.22	1.13 ± 0.29
		Macroscopic current (activation; ±SE)	Tail current (deactivation; ±SE)
mHCN2-EGFP	NSNA	2.70 ± 0.21 (n = 3)	2.29 ± 0.61 (n = 3)
mHCN2	NSNA	2.37 ± 0.24 (n = 4)	2.27 ± 0.28 (n = 4)
spHCN-EGFP	NSNA	1.03 ± 0.11 (n = 3)	0.99 ± 0.27 (n = 3)
spHCN	NSNA	0.76 ± 0.16 (n = 4)	0.49 ± 0.10 (n = 4)

SD of the PCF results was calculated using the following equation based on Eq. 4:

Δ γ_{H C N} = γ_{H C N} \cdot \sqrt{{(\frac{Δ B_{C N G}}{B_{C N G}})}^{2} + {(\frac{Δ P_{C N G}^{o}}{P_{C N G}^{o}})}^{2} + {(\frac{Δ γ_{C N G}}{γ_{C N G}})}^{2} + {(\frac{Δ B_{H C N}}{B_{H C N}})}^{2} + {(\frac{Δ P_{H C N}^{o}}{P_{H C N}^{o}})}^{2}},

where ΔB and ΔP represent the SD of the F-I slope and the open probability, respectively. Because the maximal open probability and the single-channel conductance of the CNG channel were directly derived from electrophysiology recordings, their contributions to the error should be minimal compared with the contributions by the slopes of the F-I plots and by the maximal open probability of HCN channels based on NSNA.

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