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Table 1.
Summary of the results on HCN channel conductance
HCN channelsExperimental methodResults (pS)
Pmax = 1 (±SD)Pmax from NSNA (±SD)
mHCN1-EGFP PCF 0.46 ± 0.07  
mHCN2-EGFP PCF 1.46 ± 0.26 1.71 ± 0.34 
spHCN-EGFP PCF 0.88 ± 0.22 1.13 ± 0.29 
  Macroscopic current (activation; ±SE) Tail current (deactivation; ±SE) 
mHCN2-EGFP NSNA 2.70 ± 0.21 (n = 3) 2.29 ± 0.61 (n = 3) 
mHCN2 NSNA 2.37 ± 0.24 (n = 4) 2.27 ± 0.28 (n = 4) 
spHCN-EGFP NSNA 1.03 ± 0.11 (n = 3) 0.99 ± 0.27 (n = 3) 
spHCN NSNA 0.76 ± 0.16 (n = 4) 0.49 ± 0.10 (n = 4) 
HCN channelsExperimental methodResults (pS)
Pmax = 1 (±SD)Pmax from NSNA (±SD)
mHCN1-EGFP PCF 0.46 ± 0.07  
mHCN2-EGFP PCF 1.46 ± 0.26 1.71 ± 0.34 
spHCN-EGFP PCF 0.88 ± 0.22 1.13 ± 0.29 
  Macroscopic current (activation; ±SE) Tail current (deactivation; ±SE) 
mHCN2-EGFP NSNA 2.70 ± 0.21 (n = 3) 2.29 ± 0.61 (n = 3) 
mHCN2 NSNA 2.37 ± 0.24 (n = 4) 2.27 ± 0.28 (n = 4) 
spHCN-EGFP NSNA 1.03 ± 0.11 (n = 3) 0.99 ± 0.27 (n = 3) 
spHCN NSNA 0.76 ± 0.16 (n = 4) 0.49 ± 0.10 (n = 4) 
SD of the PCF results was calculated using the following equation based on Eq. 4:
Δγ HCN =γ HCN ·( ΔB CNG B CNG ) 2 + ( ΔP CNG o P CNG o ) 2 +( Δγ CNG γ CNG ) 2 +( ΔB HCN B HCN ) 2 +( ΔP HCN o P HCN o ) 2 ,
where ΔB and ΔP represent the SD of the F-I slope and the open probability, respectively. Because the maximal open probability and the single-channel conductance of the CNG channel were directly derived from electrophysiology recordings, their contributions to the error should be minimal compared with the contributions by the slopes of the F-I plots and by the maximal open probability of HCN channels based on NSNA.
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