Skip to Main Content
Table 2.

Mobile buffer in two-buffer terminals

Buffer Kd,e1 BT,e1 Kd,e2 BT,e2 VA τ [Ca2+]Free,0 n 
 µM µM µM µM  s µM  
Immobile 0.47 ± 0.09 230 ± 90 5.5 ± 1.2 105 ± 8 0.39 ± 0.08 0.612 ± 0.08 
Mobile 0.28 ± 0.14 470 ± 210 3.5 ± 0.7 180 ± 90 0.37 ± 0.06 243 ± 56 0.603 ± 0.11 
Buffer Kd,e1 BT,e1 Kd,e2 BT,e2 VA τ [Ca2+]Free,0 n 
 µM µM µM µM  s µM  
Immobile 0.47 ± 0.09 230 ± 90 5.5 ± 1.2 105 ± 8 0.39 ± 0.08 0.612 ± 0.08 
Mobile 0.28 ± 0.14 470 ± 210 3.5 ± 0.7 180 ± 90 0.37 ± 0.06 243 ± 56 0.603 ± 0.11 

Endogenous Ca2+ buffer properties from nerve terminals with two endogenous buffers, where either both components appear to have undetectable mobility (top row) or the higher-affinity buffer has a significantly higher mobility (bottom row). Kd,e1, BT,e1, Kd,e2, BT,e2, and τ were determined by fitting to Eq. 2, with the inclusion of an exponential factor multiplying [Be1] and allowing the time constant to vary. VA and [Ca2+]Free,0 were as described in Table 1. τ gives the time constant for exponential decay of the concentration of the high-affinity endogenous buffer by diffusion into the patch pipette. For the top row, no washout could be detected. The fitting yielded a value for τ indistinguishable from zero.

Close Modal

or Create an Account

Close Modal
Close Modal