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Table I.

Slope parameters (Δα) extracted from ensemble current analyses

Constructs [Ca2+Slope parameter Δα (mean ± SD) No. of patches 
 M mmHg−1  
WT 10−3 0.015 ± 0.0043 
WT 10−5 0.016 ± 0.0050 
WT 10−6 0.018 ± 0.0031 
358–486-12GGS 10−5 0.012 ± 0.0033 
Constructs [Ca2+Slope parameter Δα (mean ± SD) No. of patches 
 M mmHg−1  
WT 10−3 0.015 ± 0.0043 
WT 10−5 0.016 ± 0.0050 
WT 10−6 0.018 ± 0.0031 
358–486-12GGS 10−5 0.012 ± 0.0033 

The ensemble current plots of Po versus pressure from wild type at different [Ca2+] (10−6, 10−5, and 10−3 M) and 358–486-12GGS at intermediate [Ca2+] (10−5 M) were fitted with the Boltzmann equation: Po = 1/[1 + a × exp(−Δα × P)] (Fig. 1 B). The slope parameters, Δα, of the wild type at different [Ca2+] are not significantly different (P = 0.38, 0.28, and 0.28 in a pairwise t test). Δα of 358–456-12GGS is also not significantly different from that of the wild type at 10−5 M Ca2+ (P = 0.07).

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