Kinetic Analyses of AChR Mutants
| Construct . | Agonist . | k0 (s−1) . | kcobs (s−1) . | kccor (s−1) . | Keq (ko/kccor) . | Normalized Keq (mut/wt) . | n . |
|---|---|---|---|---|---|---|---|
| K145C | ACh | 2030 (50) | 1200 (126) | 1500 (157) | 1.38 (0.14) | 0.05 | 3 |
| K145A | ACh | 1111 (102) | 1385 (126) | 1732 (157) | 0.65 (0.08) | 0.02 | 3 |
| K145R | ACh | 1061 (13) | 1808 (85) | 2260 (106) | 0.47 (0.02) | 0.016 | 3 |
| K145D | ACh | 210 (42) | 1603 (4) | 2003 (002) | 0.10 (0.02) | 0.004 | 2 |
| Construct . | Agonist . | k0 (s−1) . | kcobs (s−1) . | kccor (s−1) . | Keq (ko/kccor) . | Normalized Keq (mut/wt) . | n . |
|---|---|---|---|---|---|---|---|
| K145C | ACh | 2030 (50) | 1200 (126) | 1500 (157) | 1.38 (0.14) | 0.05 | 3 |
| K145A | ACh | 1111 (102) | 1385 (126) | 1732 (157) | 0.65 (0.08) | 0.02 | 3 |
| K145R | ACh | 1061 (13) | 1808 (85) | 2260 (106) | 0.47 (0.02) | 0.016 | 3 |
| K145D | ACh | 210 (42) | 1603 (4) | 2003 (002) | 0.10 (0.02) | 0.004 | 2 |
All the mutants tested at position K145 produced loss of function constructs. The abbreviations used here are the same as indicated earlier.