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TABLE V

Kinetic Analyses of AChR Mutants

Construct
Agonist
k0 (s−1)
kcobs (s−1)
kccor (s−1)
Keq (ko/kccor)
Normalized Keq (mut/wt)
n
K145C ACh 2030 (50) 1200 (126) 1500 (157) 1.38 (0.14) 0.05 
K145A ACh 1111 (102) 1385 (126) 1732 (157) 0.65 (0.08) 0.02 
K145R ACh 1061 (13) 1808 (85) 2260 (106) 0.47 (0.02) 0.016 
K145D ACh 210 (42) 1603 (4) 2003 (002) 0.10 (0.02) 0.004 
Construct
Agonist
k0 (s−1)
kcobs (s−1)
kccor (s−1)
Keq (ko/kccor)
Normalized Keq (mut/wt)
n
K145C ACh 2030 (50) 1200 (126) 1500 (157) 1.38 (0.14) 0.05 
K145A ACh 1111 (102) 1385 (126) 1732 (157) 0.65 (0.08) 0.02 
K145R ACh 1061 (13) 1808 (85) 2260 (106) 0.47 (0.02) 0.016 
K145D ACh 210 (42) 1603 (4) 2003 (002) 0.10 (0.02) 0.004 

All the mutants tested at position K145 produced loss of function constructs. The abbreviations used here are the same as indicated earlier.

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