Effects of SGTx Mutations on Interactions of the Toxin with Anionic Membranes
SGTx1 . | Kx . | ΔΔGP . | ΔΔGO . |
---|---|---|---|
kcal mol−1 | kcal mol−1 | ||
WT | 3.4 × 106 | – | – |
T1A | 1.3 × 106 | 0.6 | 0.0 |
R3A | 7.0 × 105 | 1.0 | >3.0 |
Y4A | 1.5 × 105 | 1.9 | 1.2 |
L5A | 4.9 × 105 | 1.1 | >3.0 |
F6A | 6.1 × 105 | 1.0 | >3.4 |
G7A | 1.3 × 106 | 0.6 | 0.8 |
G8A | 1.8 × 106 | 0.4 | 0.2 |
K10A | 6.8 × 105 | 1.0 | 0.4 |
T12A | 6.8 × 105 | 1.0 | 0.2 |
A13S | 7.7 × 105 | 0.9 | 0.1 |
D14A | 6.5 × 106 | −0.4 | −1.1 |
K17A | 6.1 × 105 | 1.0 | 0.3 |
H18A | 2.7 × 105 | 1.5 | 1.2 |
A20S | 9.7 × 105 | 0.7 | 0.7 |
R22A | 7.9 × 105 | 0.7 | >3.0 |
S23A | 1.3 × 106 | 0.6 | 0.4 |
D24A | 1.0 × 107 | −0.6 | −1.8 |
G25A | 1.6 × 106 | 0.4 | 0.4 |
K26A | 3.9 × 105 | 1.3 | 0.5 |
A29S | 7.4 × 105 | 0.9 | 1.2 |
D31A | 1.5 × 106 | 0.5 | 1.4 |
G32A | 6.4 × 105 | 1.0 | 0.8 |
T33A | 6.7 × 105 | 1.0 | 0.8 |
F34A | 2.3 × 105 | 1.6 | 0.7 |
SGTx1 . | Kx . | ΔΔGP . | ΔΔGO . |
---|---|---|---|
kcal mol−1 | kcal mol−1 | ||
WT | 3.4 × 106 | – | – |
T1A | 1.3 × 106 | 0.6 | 0.0 |
R3A | 7.0 × 105 | 1.0 | >3.0 |
Y4A | 1.5 × 105 | 1.9 | 1.2 |
L5A | 4.9 × 105 | 1.1 | >3.0 |
F6A | 6.1 × 105 | 1.0 | >3.4 |
G7A | 1.3 × 106 | 0.6 | 0.8 |
G8A | 1.8 × 106 | 0.4 | 0.2 |
K10A | 6.8 × 105 | 1.0 | 0.4 |
T12A | 6.8 × 105 | 1.0 | 0.2 |
A13S | 7.7 × 105 | 0.9 | 0.1 |
D14A | 6.5 × 106 | −0.4 | −1.1 |
K17A | 6.1 × 105 | 1.0 | 0.3 |
H18A | 2.7 × 105 | 1.5 | 1.2 |
A20S | 9.7 × 105 | 0.7 | 0.7 |
R22A | 7.9 × 105 | 0.7 | >3.0 |
S23A | 1.3 × 106 | 0.6 | 0.4 |
D24A | 1.0 × 107 | −0.6 | −1.8 |
G25A | 1.6 × 106 | 0.4 | 0.4 |
K26A | 3.9 × 105 | 1.3 | 0.5 |
A29S | 7.4 × 105 | 0.9 | 1.2 |
D31A | 1.5 × 106 | 0.5 | 1.4 |
G32A | 6.4 × 105 | 1.0 | 0.8 |
T33A | 6.7 × 105 | 1.0 | 0.8 |
F34A | 2.3 × 105 | 1.6 | 0.7 |
ΔΔGO values are from Wang et al. (2004).