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TABLE I

Kinetic Analysis of the Experimental Data under Scheme 1


a. Split Scheme 1 analysis
b. Global Scheme 1 analysis
Macroscopic parameters   
τrise (ms) 0.518 ± 10.0% 0.788a 
τdecay (ms) 61.3 ± 17.2% 56.3 
tδ (ms) 0.203 ± 0.007 0.094a 
Am 0.714 ± 0.018 0.68 
ka (s−1 μM−16.09 ± 5.7% 6.29 
EC50 (μM) 16.3 14.9 
kinetic rates   
kon (s−1 μM−16.09 ± 5.7% 6.73 
koff (s−1212 ± 12.5% 166 
α (s−154.3 ± 19.8% 45.3 
β (s−11681 ± 12.5% 743 
Microscopic constants   
Π 0.690 0.600 
Km (μM) 31.7 24.6 
24.2 16.4 

a. Split Scheme 1 analysis
b. Global Scheme 1 analysis
Macroscopic parameters   
τrise (ms) 0.518 ± 10.0% 0.788a 
τdecay (ms) 61.3 ± 17.2% 56.3 
tδ (ms) 0.203 ± 0.007 0.094a 
Am 0.714 ± 0.018 0.68 
ka (s−1 μM−16.09 ± 5.7% 6.29 
EC50 (μM) 16.3 14.9 
kinetic rates   
kon (s−1 μM−16.09 ± 5.7% 6.73 
koff (s−1212 ± 12.5% 166 
α (s−154.3 ± 19.8% 45.3 
β (s−11681 ± 12.5% 743 
Microscopic constants   
Π 0.690 0.600 
Km (μM) 31.7 24.6 
24.2 16.4 

Values of macroscopic parameters, kinetic rates, and microscopic constants obtained from six patches by using a split analysis or a global analysis. In the first case we obtained the macroscopic parameters from the experimental data (in bold); the kinetic rates were calculated from the macroscopic parameters. In the second case we obtained the kinetic rates directly from the experimental data (starting 0.5 ms after the application of the ATP pulse) by running a nonlinear algorithm. The estimated rates from this fit are shown (in bold). The macroscopic parameters and the microscopic constants were calculated from these kinetic rates.

a

Parameter that differs significantly between the two fits.

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