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TABLE I

Time Constants (τ) and Amplitudes (A) for Current (I) and Fluorescence (F) from Experiments Similar to Fig. 5 

Residue
τ1I
τ2I
A1/(A1+A2)I
τ1F
τ2F
A1/(A1+A2)F
n
327C 1238 ± 190 N/A N/A 884 ± 157 N/A N/A 
328C 38 ± 5 334 ± 22 0.59 ± 0.07 9.5 ± 1.5 631 ± 109 0.49 ± 0.04 
329C N/A N/A N/A N/A N/A N/A  
330C 152 ± 32 2696 ± 476 0.46 ± 0.05 5.7 ± 2.1 4919 ± 1091 0.06 ± 0.02 
331C 24 ± 1 396 ± 42 0.19 ± 0.04 4.5 ± 0.2 122 ± 7 0.77 ± 0.02 
332C 18 ± 5 135 ± 31 0.46 ± 0.09 5.9 ± 0.8 62 ± 5 0.27 ± 0.02 
Residue
τ1I
τ2I
A1/(A1+A2)I
τ1F
τ2F
A1/(A1+A2)F
n
327C 1238 ± 190 N/A N/A 884 ± 157 N/A N/A 
328C 38 ± 5 334 ± 22 0.59 ± 0.07 9.5 ± 1.5 631 ± 109 0.49 ± 0.04 
329C N/A N/A N/A N/A N/A N/A  
330C 152 ± 32 2696 ± 476 0.46 ± 0.05 5.7 ± 2.1 4919 ± 1091 0.06 ± 0.02 
331C 24 ± 1 396 ± 42 0.19 ± 0.04 4.5 ± 0.2 122 ± 7 0.77 ± 0.02 
332C 18 ± 5 135 ± 31 0.46 ± 0.09 5.9 ± 0.8 62 ± 5 0.27 ± 0.02 

Voltage step to −120 mV. τ in ms. For residue 327, I and F were well fit with a single exponential at −120 mV. For residues 328–332, two exponentials were needed to get good fits. 329C did not express well enough to give a clear fluorescence signal.

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