Skip to Main Content
TABLE I

Cd2+ Modification of I470C

Open Statea
 
  
    kon (104 M−1s−13.36 ± 0.25 (n = 4)  
    koff (s−10.18 ± 0.03 (n = 4)  
    Kd (μM) 5.47  
Inactivated State   
    kon (104 M−1s−14.8 ± 0.2 (n = 15)b 5.2d (n = 1) 
    koff (s−10.083 ± 0.004 (n = 15)b 0.072 ± 0.002c (n = 16) 
    Kd (μM) 1.7b 1.4bc 
Ratio of O/I   
    kon 0.70 ± 0.06 0.65 
    koff 2.22 ± 0.36 2.53 ± 0.39 
    Kd 3.21 3.9 
Open Statea
 
  
    kon (104 M−1s−13.36 ± 0.25 (n = 4)  
    koff (s−10.18 ± 0.03 (n = 4)  
    Kd (μM) 5.47  
Inactivated State   
    kon (104 M−1s−14.8 ± 0.2 (n = 15)b 5.2d (n = 1) 
    koff (s−10.083 ± 0.004 (n = 15)b 0.072 ± 0.002c (n = 16) 
    Kd (μM) 1.7b 1.4bc 
Ratio of O/I   
    kon 0.70 ± 0.06 0.65 
    koff 2.22 ± 0.36 2.53 ± 0.39 
    Kd 3.21 3.9 
a

Determined from τmod = 1/(kon + koff) and R = koff /(kon + koff), as described in the text.

b

Values were determined from an analytical fit considering binding and unbinding of Cd2+ during periods I–III, as described in the text and Fig. 7 A.

c

Values were determined from washout experiments as described in text and Fig. 6.

d

Values were determined from “single period” experiment (Fig. 7 B).

Close Modal

or Create an Account

Close Modal
Close Modal