Composition of Intracellular Solutions in mM
. | Free Ca . | 0 Ca . | 30 nM Ca . | 100 nM Ca . | 150 nM Ca . | 300 nM Ca . | 500 nM Ca . | 1000 nM Ca . |
---|---|---|---|---|---|---|---|---|
BAPTA | 0 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
CaCl2 | 0 | 0 | 3.1 | 4 | 5.7 | 6.87 | 8.2 | |
K-glutamate | 140 | 120 | 120 | 120 | 120 | 120 | 120 | 120 |
NaCl | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 |
MgCl2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
HEPES | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
. | Free Ca . | 0 Ca . | 30 nM Ca . | 100 nM Ca . | 150 nM Ca . | 300 nM Ca . | 500 nM Ca . | 1000 nM Ca . |
---|---|---|---|---|---|---|---|---|
BAPTA | 0 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
CaCl2 | 0 | 0 | 3.1 | 4 | 5.7 | 6.87 | 8.2 | |
K-glutamate | 140 | 120 | 120 | 120 | 120 | 120 | 120 | 120 |
NaCl | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 |
MgCl2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
HEPES | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
pH 7.2 (adjusted with NaOH or HCl); osmolarity, 300–310 mOsm (adjusted with sucrose). WebMax parameters: temperature = 24°C, pH = 7.2, ionic strength = 0.16.