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TABLE III

Response Parameters of RhEQ/mG Rods for Flashes at Each Wavelength (n = 14)


500 nm pre
600 nm
652 nm
676 nm
500 nm post
RhEQ: mG (%) 90: 10 31: 69 16: 84 12: 88 90: 10 
Dark current (pA) 13.0 ± 0.7 13.3 ± 0.6 13.4 ± 0.5 13.6 ± 0.6 13.6 ± 0.6 
Time to peak (ms) 145 ± 4 150 ± 6 146 ± 6 145 ± 5 151 ± 9 
Integration time (ms) 276 ± 18 233 ± 18a 228 ± 17a 228 ± 14a 289 ± 18 
σ2/ m (pA) 0.48 ± 0.02 0.40 ± 0.03a 0.36 ± 0.03a 0.30 ± 0.03b 0.47 ± 0.03 

500 nm pre
600 nm
652 nm
676 nm
500 nm post
RhEQ: mG (%) 90: 10 31: 69 16: 84 12: 88 90: 10 
Dark current (pA) 13.0 ± 0.7 13.3 ± 0.6 13.4 ± 0.5 13.6 ± 0.6 13.6 ± 0.6 
Time to peak (ms) 145 ± 4 150 ± 6 146 ± 6 145 ± 5 151 ± 9 
Integration time (ms) 276 ± 18 233 ± 18a 228 ± 17a 228 ± 14a 289 ± 18 
σ2/ m (pA) 0.48 ± 0.02 0.40 ± 0.03a 0.36 ± 0.03a 0.30 ± 0.03b 0.47 ± 0.03 

RhEQ: mG represents the predicted percentages of E122Q rhodopsin and mouse green photoactivated at each wavelength in RhEQ/mG rods (see Results). Response parameters were obtained from 14 RhEQ/mG rods, for each of which stimuli of 500, 600, 652, 676, and 500-nm flashes were delivered in the order. The 500-nm flashes used in the first and the end of the experiment are referred as 500 nm pre and 500 nm post, respectively. The kinetics parameters of single-photon response were taken from dim flash responses, the average of which is <0.2rmax. σ2/m is the ensemble variance-to-mean ratio of dim flash stimuli (>30 times) for each wavelength. Values are means ± SEM.

a

Difference from 500-nm pre flashes being statistically significant, P < 0.05.

b

Difference from 500-nm pre flashes being statistically significant, P < 0.005. The statistical significance was determined by using the two-tailed paired Student's t test.

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